T5 Exonuclease | 貨號E089 1000 U (100 µl)

abm提供了一系列核酸酶 nucleases)，可製備RNA和DNA，以便在下一代全基因組測序 (whole genome sequencing)和文庫構建(library construction)中用於後續應用。

T5核酸外切酶(T5 exonuclease)5’至3’方向上5’末端或線性或環狀dsDNA切口處核苷酸的降解。該外切核酸酶在鎂離子存在下也具有ssDNA核酸內切酶活性，但不會降解超螺旋dsDNA(upercoiled dsDNA)

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Description	T5 exonuclease catalyzes the degradation of nucleotides either from the 5’ termini or at nicks of linear or circular dsDNA in a 5’ to 3’ direction. This exonuclease also exhibits ssDNA endonuclease activity in the presence of magnesium ions, but will not degrade supercoiled dsDNA.
SKU	E089
Unit quantity	1000 U (100 µl)
Applications	Degradation of ssDNA, dsDNA linear, circular or nicked, while preserving supercoiled plasmid DNA. Removal of contaminant denatured DNA isolated during alkaline lysis plasmid purification. Generation of 3’ overhangs for cloning procedures.
Concentration	10000 U/ml
Format	Enzyme supplied with a 10X Reaction Buffer. Incubate at 37°C.
Storage Condition	Store all components at -20°C.
Storage Buffer	50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol and 0.1% Triton X-100.
Note	One unit is defined as the amount of enzyme necessary to catalyze the release of 1 nmol of acid-soluble nucleotides from double-stranded DNA in a 50 µl volume in 30 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use.