Goat IgG-UNLB 同型對照組 / 分型對照組  | 貨號0109-01 |規格 5.0 mg/mL (體積2.0ml)

Isotype control的目的: 確定一抗的結合是否特異性；而不是非特異性的Fc受體或與其他蛋白的相互作用所造成的Signal。還可以用來競爭性的結合抗體，與功能阻斷抗體發揮同樣的功能。Isotype control應用於流氏細胞儀抗體分型control、ELISA抗體分型control、FLISA抗體分型control 、IHC抗體分型control 、IP抗體分型control 、invitro 抗體分型control 、invivo抗體分型control

使用Isotype control的原則: 同型對照/分型對照要與一抗的來源，Ig分型和標記完全一致。同型對照/分型對照（Isotype Control）：使用與一抗相同種屬來源、相同亞型、相同劑量和相同的免疫球蛋白及亞型的免疫球蛋白，用於消除由於抗體非特異性結合到細胞表面而產生的背景染色。如果一抗是多抗，可以用an normal serum（與一抗相同的正常血清），此方法常在IHC染色中常被用於負對照組；假若多重螢光染色，由於螢光標記單抗的來源不同，應選用相同來源的未標記單抗作為同型對照來 調整背景染色。

例子說明: 比如檢測一抗為單抗的mouse anti rat CD11b，clone OX-42 purified IgG，那麼它的isotype 是mouse IgG2a， 所以可以用purified（純化的） mouse IgG2a來做OX-42的同型對照（Isotype Control）。

細胞免疫專家極力推薦SouthernBiotech高品質Isotype control

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質Isotype control-免疫球蛋白系列產品，具有螢光物質（FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）各種螢光標誌、另具酵素（HRP、AP）、或未標誌及Low Endotoxin/Azide Free（LE/AF）等標定。

Source	Normal goat serum
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2
Concentration	5.0 mg/mL
Volume	2.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include – Flow Cytometry 1-4 ELISA FLISA Other referenced applications for relevant formats include – Immunohistochemistry-Frozen Sections 5-7 Immunohistochemistry-Paraffin Sections 8 Immunohistochemistry-Whole Mount 18 Immunocytochemistry 19,20 Immunoprecipitation 21 Blocking 4,9,10 In vitro Control 11-15 In vivo Control 16,17
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2793944

Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD57-UNLB followed by Goat IgG-UNLB (SB Cat. No. 0109-01; gray) and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01) then Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).

CEM/LAV-1 cell lysate was immunoprecipitated with anti-GAPDH or Goat IgG-UNLB (SB Cat. No. 0109-01). Protein was resolved by electrophoresis, transferred to membrane, and probed with anti-GAPDH followed by a secondary antibody and chemiluminescent detection.

Image from Kishimoto N, Onitsuka A, Kido K, Takamune N, Shoji S, Misumi S. Glyceraldehyde 3-phosphate dehydrogenase negatively regulates human immunodeficiency virus type 1 infection. Retrovirology. 2012;9:107. Figure 3(d)

References

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2. Pospisil R, Young-Cooper GO, Mage RG. Preferential expansion and survival of B lymphocytes based on VH framework 1 and framework 3 expression: “positive” selection in appendix of normal and VH-mutant rabbits. Proc Natl Acad Sci USA. 1995;92:6961-5. (FC)
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7. Menke J, Zeller GC, Kikawada E, Means TK, Huang XR, Lan HY, et al. CXCL9, but not CXCL10, promotes CXCR3-dependent immune-mediated kidney disease. J Am Soc Nephrol. 2008;19:1177-89. (IHC-FS)
8. Tamura M, Aizawa R, Hori M, Ozaki H. Progressive renal dysfunction and macrophage infiltration in interstitial fibrosis in an adenine-induced tubulointerstitial nephritis mouse model. Histochem Cell Biol. 2009;131:483-90. (IHC-PS)
9. Boesze-Battaglia K, Besack D, McKay T, Zekavat A, Otis L, Jordan-Sciutto K, et al. Cholesterol-rich membrane microdomains mediate cell cycle arrest induced by Actinobacillus actinomycetemcomitans cytolethal-distending toxin. Cell Microbiol. 2006;8:823-36. (Block)
10. Pepin AC, Tandon R, Cattori V, Niederer E, Riond B, Willi B, et al. Cellular segregation of feline leukemia provirus and viral RNA in leukocyte subsets of long-term experimentally infected cats. Virus Res. 2007;127:9-16. (Block)
11. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (In vitro control)
12. Sato H, Wang D, Kudo A. Dissociation of Pax-5 from KI and KII sites during κ-chain gene rearrangement correlates with its association with the underphosphorylated form of retinoblastoma. J Immunol. 2001;166:6704-10. (In vitro control)
13. Petlickovski A, Laurenti L, Li X, Marietti S, Chiusolo P, Sica S, et al. Sustained signaling through the B-cell receptor induces Mcl-1 and promotes survival of chronic lymphocytic leukemia B cells. Blood. 2005;105:4820-7. (In vitro control)
14. Zizzo G, Hilliard BA, Monestier M, Cohen PL. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20. (In vitro control)
15. Li H, Usas A, Poddar M, Chen C, Thompson S, Ahani B, et al. Platelet-rich plasma promotes the proliferation of human muscle derived progenitor cells and maintains their stemness. PLoS One. 2013;8(6):e64923. (In vitro control)
16. Lo JC, Chin RK, Lee Y, Kang H, Wang Y, Weinstock JV, et al. Differential regulation of CCL21 in lymphoid/nonlymphoid tissues for effectively attracting T cells to peripheral tissues. J Clin Invest. 2003;112:1495-505. (In vivo control)
17. Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1β. Genes Dev. 2006;20:2527-38. (In vivo control)
18. Lämmermann T, Afonso PV, Angermann BR, Wang JM, Kastenmüller W, Parent CA, et al. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature. 2013;498:371-5. (IHC-WM)
19. Hauser SP, Waldron JA, Upuda KB, Lipschitz DA. Morphological characterization of stromal cell types in hematopoietically active long-term murine bone marrow cultures. J Histochem Cytochem. 1995;43:371-9. (ICC)
20. Hsieh-Bonassera ND, Wu I, Lin JK, Schumacher BL, Chen AC, Masuda K, et al. Expansion and redifferentiation of chondrocytes from osteoarthritic cartilage: cells for human cartilage tissue engineering. Tissue Eng Part A. 2009;15:3513-23. (ICC)
21. Kishimoto N, Onitsuka A, Kido K, Takamune N, Shoji S, Misumi S. Glyceraldehyde 3-phosphate dehydrogenase negatively regulates human immunodeficiency virus type 1 infection. Retrovirology. 2012;9:107. (IP)