TNF-alpha 重組蛋白 | Recombinant Mouse TNF (E. coli) ABM貨號Z200219

TNF在被細菌脂多醣刺激後由巨噬細胞(macrophages)，單核細胞(monocytes)，嗜中性粒細胞(neutrophils)，T細胞(T cells)，自然殺傷細胞(natural killer cells )分泌。 表達CD4的細胞分泌TNF-α，而CD8（+）細胞則分泌很少或不分泌TNF-α。 受刺激的外周嗜中性粒細胞(neutrophilic granulocytes)也受刺激的細胞，以及許多轉化的細胞株，星形膠質細胞(astrocytes)，小膠質細胞(microglial cells)，平滑肌細胞( smooth muscle cells)和成纖維細胞(fibroblasts )也分泌TNF。 人乳中也含有該因子。 TNF-α的合成由許多不同的刺激誘導，包括干擾素(interferons)，IL-2，GM-CSF，SP，緩激肽(Bradykinin)，免疫複合物(immune complexes)，環氧合酶抑制劑(cyclooxygenase)和PAF（血小板活化因子）。 人TNF-α是17 kDa的非糖基化蛋白，長度為157個氨基酸。 鼠TNF-α是N-糖基化的。 TNF-β的同源性約為30％。 TNF-α形成二聚體和三聚體。

Description	TNF is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF-alpha while CD8(+) cells secrete little or no TNF-alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2, GM-CSF, SP, Bradykinin, immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor). Human TNF-alpha is a non-glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF-alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF-alpha forms dimers and trimers.
SKU	Z200219
Growth Factor Size	1.0 mg
Unit quantity	1.0 mg
Family	TNF Super-family
Aliases	TNF-alpha, TNF-a, Tumor necrosis factor ligand superfamily member 2, Cachectin, Tnfa, Tnfsf2, Tnf Recombinant Mouse Tumor Necrosis Factor Alpha (TNF)
Gene Symbol	TNF
Gene ID	21926
Accession Number	P06804
Source	E. coli
Species	Mouse (M. musculus)
Appearance	Lyophilized Powder
Molecular Weight	17 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant mouse TNF alpha was lyophilized from 0.2 μm filtered PBS solution, pH 7.0.
Function	The ED(50) was determined cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 3.0 x 10^7 units/mg.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° – 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.