Human Enolase 1 (ENO1)試劑盒 | Human Enolase 1 (ENO1) ELISA Kit貨號KTE63925
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Human Enolase 1 (ENO1)試劑盒 | Human Enolase 1 (ENO1) ELISA Kit貨號KTE63925
產品描述：Enolase 1 (ENO1)ELISA試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的Enolase 1 (ENO1)。對Enolase 1 (ENO1)特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中，並且存在的任何Enolase 1 (ENO1)都被固定的抗體結合。除去所有未結合的物質後，將HRP-綴合Enolase 1 (ENO1)檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後，將色原溶液添加到孔中，並且根據在初始步驟中結合的Enolase 1 (ENO1)的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍0.25 ng/mL – 4 ng/mL，最低靈敏度0.01ng/mL可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum
- Product name
Human Enolase 1 (ENO1) ELISA Kit
Reactivity Human ＿
- Applications
ELISA
- Detection method
Colorimetric
- Sample type
Cell culture supernatants, Other biological fluids, Plasma, Serum
- Assay type
Sandwich ELISA (quantitative)
- Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
- Kit components
- Human Enolase 1 (ENO1) microplate
• Human Enolase 1 (ENO1) standard
• Human Enolase 1 (ENO1) detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers - Features & Benefits
Human Enolase 1 (ENO1) ELISA Kit has high sensitivity and excellent specificity for detection of Human Enolase 1 (ENO1) ELISA Kit. No significant cross-reactivity or interference between Human Enolase 1 (ENO1) ELISA Kit and analogues was observed.
- Calibration range
0.25 – 4 ng/mL Limit of detection 0.01 ng/mL
- Usage notes
- Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
使用注意事項
- 請勿混用不同試劑盒中的組分，或在試劑盒到期日期之後使用試劑。
- 打開之前，讓所有試劑至少加室溫30分鐘。
- 用試劑預清洗移液器吸頭，為每個樣品，標準液和試劑使用新鮮的移液器吸頭，以免污染。
- 未使用的孔必須在提供的密封袋中於4°C乾燥。
- 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
- 建議所有樣品和標準品一式兩份或一式三份進行測定。