人類晚期糖基化終產物（AGEs）ELISA試劑盒 | Human Advanced glycation end products (AGEs) ELISA Kit貨號KTE60961

Human Advanced glycation end products (AGEs) ELISA Kit

產品描述：人類晚期糖基化終產物（AGEs）ELISA試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的人類晚期糖基化終產物（AGEs）。人類晚期糖基化終產物（AGEs）特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中，並且存在的任何纖維蛋白原都被固定的抗體結合。除去所有未結合的物質後，將HRP-綴合人類晚期糖基化終產物（AGEs）檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後，將色原溶液添加到孔中，並且根據在初始步驟中結合的人類晚期糖基化終產物（AGEs）的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 30 pg/mL-480 pg/mL, 最低靈敏度1.0 pg/mL。可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

• Product name

Human Advanced glycation end products (AGEs) ELISA Kit

• Reactivity

Human

• Applications

ELISA

• Application notes

Human Advanced glycation end products (AGEs) ELISA Kit employs a two-site sandwich ELISA to quantitate AGEs in samples. An antibody specific for AGEs has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGEs present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human AGEs detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of AGEs bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Detection method

Colorimetric

• Sample type

Cell culture supernatants, Other biological fluids, Plasma, Serum

• Assay type

Sandwich ELISA

• Calibration range

30 pg/mL-480 pg/mL

• Limit of detection

1.0 pg/mL

使用注意事項

• 請勿混用不同試劑盒中的組分，或在試劑盒到期日期之後使用試劑。
• 打開之前，讓所有試劑至少加室溫30分鐘。
• 用試劑預清洗移液器吸頭，為每個樣品，標準液和試劑使用新鮮的移液器吸頭，以免污染。
• 未使用的孔必須在提供的密封袋中於4°C乾燥。
• 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
• 建議所有樣品和標準品一式兩份或一式三份進行測定。