大鼠胰島素樣生長因數1(IGF-1) ELISA試劑盒| Rat Insulin-like growth factor 1 (IGF-1) ELISA Kit貨號KTE100729

偵測範圍: 1.875ng/mL-30ng/mL, 最低靈敏度0.1 ng/mL

產品描述：大鼠胰島素樣生長因數1(IGF-1)ELISA套件採用採用兩點夾心ELISA(Sandwich ELISA) 來定量樣品中的IGF-1。IGF-1特有的抗體已預先塗覆到微孔板上。標準和樣品移入Well, 任何IGF-1存在都受固定抗體的約束。去除任何未結合的物質後,HRP-結合RatIGF-1檢測抗體被添加到Well中。洗滌去除任何未綁定的 HRP 試劑,將色變溶液添加到Well中,顏色與初始步驟中 IGF-1 綁定量成比例發展。停止顯色並測量顏色的強度。偵測範圍: 1.875ng/mL-30ng/mL, 最低靈敏度0.1 ng/mL。可應用於以下樣品來源: Other biological fluids, Plasma, Serum

• Product nameRat Insulin-like growth factor 1 (IGF-1) ELISA Kit
• ReactivityRat
• ApplicationsELISA
• Application notesRat Insulin-like growth factor 1 (IGF-1) ELISA Kit employs a two-site sandwich ELISA to quantitate IGF-1 in samples. An antibody specific for IGF-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF-1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate RatIGF-1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of IGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Detection methodColorimetric
• Sample typeOther biological fluids, Plasma, Serum
• Assay typeSandwich ELISA (quantitative)
• Assay duration Multiple steps standard sandwich ELISA assay with a working time of 2-61 hours. It depends on the experience of the operation person.

使用注意事項

• 請勿混用不同試劑盒中的組分，或在試劑盒到期日期之後使用試劑。
• 打開之前，讓所有試劑至少加室溫30分鐘。
• 用試劑預清洗移液器吸頭，為每個樣品，標準液和試劑使用新鮮的移液器吸頭，以免污染。
• 未使用的孔必須在提供的密封袋中於4°C乾燥。
• 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
• 建議所有樣品和標準品一式兩份或一式三份進行測定。

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