GFP螢光蛋白定量套組 | GFP Quantity Kit貨號K815-100 (ABCAM ab235672)

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GFP螢光蛋白定量套組 | 貨號K815-100 (ABCAM ab235672)
GFP Quantity Kit (0.01-10 µg / ml)
產品描述:. 綠色螢光蛋白(Green Fluorescent Protein , GFP)是一種自發的螢光蛋白,最初是從維多利亞水母水母(Aequorea Victoria)分離得到的(Chalfie等,1994)。 GFP也已被重組增強修飾(稱為enhanced Green Fluorescent Protein, EGFP)。 GFP(Green Fluorescent Protein , GFP)和EGFP已被廣泛用作有價值的報告分子,用於體內可視化各種細胞類型和生物體中的基因表達。由於GFP不需要其他底物或輔助因子,因此可以在螢光顯微鏡下輕鬆檢測GFP(Green Fluorescent Protein , GFP)螢光。但是,大多數GFP(Green Fluorescent Protein , GFP)的影像學研究僅是定性的。由於對細胞或組織中GFP(Green Fluorescent Protein , GFP)表達水平進行定量分析的信息量更大並且具有廣泛的應用,因此BioVision已開發出GFP Quantity Kit,該試劑盒可以以96微孔板的形式定量GFP(Green Fluorescent Protein , GFP)。細胞或組織可以直接在GFP(Green Fluorescent Protein , GFP)分析緩衝液中勻漿。通過將其螢光與GFP標準品的螢光相比較來確定GFP(Green Fluorescent Protein , GFP)的量。該試劑盒可檢測多種GFP濃度(0.01-10 µg / ml)。還提供了GFP淬滅溶液,用於確定細胞或組織提取物的自發螢光。每個試劑盒提供足夠的試劑來執行多達100個測定,Ex / Em = 488/507 nm。
引用文獻
Baets et al., Defects of mutant DNMT1 are linked to a spectrum of neurological disorders. Brain, Apr 2015; 138: 845 – 861.
Chenguang Fan. et.al Short N-terminal sequences package proteins into bacterial microcompartments PNAS, Apr 2010; 107: 7509 – 7514.
Cat # +Size | K815-100 (ABCAM ab235672) |
Size | 100 assays |
Detection Method | Fluorescence (Ex/Em 488/507 nm) |
Species Reactivity | Mammalian |
Applications | It detects a wide range of GFP concentration (0.01-10 ug/ml) |
Features & Benefits | • Simple procedure; takes ~ 20 minutes • Fast and convenient |
Kit Components | • GFP Assay Buffer • GFP Standard (1 µg/µl) • GFP Quench Solution |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans |
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