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脯氨酸檢測試劑盒 | 貨號K2020-100

PicoProbe™ Proline Assay Kit (Fluorometric)

產品描述：脯氨酸(Proline)是一種蛋白質氨基酸，在蛋白質折疊(protein folding)中起重要作用。在人類中，脯氨酸(Proline)可以由谷氨酸(glutamate)和精氨酸(arginine)合成。脯氨酸(Proline)是人類有條件的必需氨基酸(essential amino acids)之一。蜜蜂 (honey bees)將花蜜(nectar)加工成蜂蜜時，也會產生脯氨酸(Proline)。因此，其在蜂蜜中的含量通常被用作蜂蜜成熟度(honey ripeness)和糖摻假(sugar adulteration)的指標(indicator)。對於成熟的蜂蜜，將180 mg脯氨酸/ kg的蜂蜜用作國際標準。測定脯氨酸含量在植物中的生物學重要性，在植物中它還可以作為stress標記(stress marker)。Unstressed的植物每克植物組織含有約0.5 µmol脯氨酸，而stressed植物的脯氨酸含量比Unstressed植物高100倍。 BioVision的PicoProbe™脯氨酸檢測試劑盒提供了一種快速，特異性和簡便的方法來測量多種樣品中脯氨酸的濃度。在該測定中，脯氨酸被轉化為中間體，該中間體將進一步與探針反應以產生強螢光信號（Ex / Em = 535/587 nm）。該工具包簡單，易於使用，靈敏且具有高通量適應性。每孔可檢測到低至5 pmol的脯氨酸。。此產品需由儀器進行定量 ELISA儀器設備請按此 

Cat # +Size	K2020-100
Size	100 assays
Kit Summary	BioVision’s PicoProbe™ Proline Assay Kit provides a quick, specific and easy method for measuring proline concentrations in a wide variety of samples. In this assay, proline is converted to an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 5 pmol of proline per well.
Detection Method	Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity	All
Applications	• Assaying proline concentration as a stress factor in plants. • Analyzing maturation of honey. • Measurement of proline in biological samples and honey.
Features & Benefits	• Specific • Easy method for measuring Proline concentrations in a wide variety of samples. • Quick
Kit Components	Proline Assay Buffer PicoProbe™ Proline Enzyme Mix Proline Cofactor Mix Proline Developer Mix Proline Standard
Storage Conditions	-20°C
Shipping Conditions	Gel Pack

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