GluTracker™葡萄糖攝取測定試劑盒（細胞） | 貨號K681-50

GluTracker™ Glucose Uptake Assay Kit (Cell-Based)

產品介紹: 葡萄糖是大多數生物中普遍存在的能源，在細胞代謝和體內平衡中起著關鍵作用。癌細胞表現出增加的葡萄糖攝取以支持其高增殖率。在BioVision，我們開發了一種螢光葡萄醣類似物，就像葡萄糖可以被細胞通過葡萄糖轉運蛋白吸收一樣。但是，這種葡萄醣類似物由於其修飾而不能完全用於糖酵解中並因此積累在細胞內。該螢光葡萄醣類似物產生的螢光與細胞攝取的葡萄糖成正比，可用於通過螢光顯微鏡和流式細胞術測量葡萄糖的攝取。為了驗證該檢測方法，該試劑盒中包含了phreretin，一種抑制葡萄糖攝取的天然酚。這種易於使用的非放射性試劑盒可對成像細胞對胰島素，生長因子等的反應攝取的葡萄糖進行成像和準確測量。

Cat # +Size	K681-50
Size	50 assays
Detection Method	FACS (488 nm excitation laser) and fluorescent microscope
Applications	Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc. Dual-staining of glucose transporters and glucose uptake Analysis of glucose metabolism and cell signaling in various cell types Screening of anti-diabetic compounds
Features & Benefits	• Easy-to-use • Non-radioactive • Image and accurately measure glucose uptake in cultured cells in response to insulin, growth factors etc.
Kit Components	• Analysis Buffer (50X)• GluTracker Reagent (100X)• GluTracker Enhancer• Phloretin (100X)
Storage Conditions	-20°C
Shipping Conditions	Gel Pack