表皮生長因子(EGF),人類重組蛋白 | 貨號4022
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Epidermal Growth Factor (EGF), human recombinant
產品介紹: 人表皮生長因子(Human epidermal growth factor , EGF)也稱為HOMG4和URG,是一種通過與受體EGFR結合而在調節細胞生長(cell growth),增殖(proliferation)和分化(differentiation)中起重要作用的生長因子。表皮生長因子(Epidermal growth factor)存在於人類血小板(human platelets),巨噬細胞(macrophages),尿液(urine),唾液(saliva),牛奶(milk)和血漿(plasma)中。 EGF是蛋白質EGF家族(EGF-family)的創始成員。該蛋白質家族的成員具有高度相似的結構和功能特徵。所有家族成員均包含一個或多個保守氨基酸序列的重複。唾液EGF的生物學作用包括口腔和胃食管潰瘍的癒合,胃酸分泌的抑制,DNA合成的刺激以及粘膜保護免受腔內傷害因素(如胃酸,膽汁酸,胃蛋白酶和胰蛋白酶)以及物理,化學作用的影響。和細菌劑。由於EGF致癌的風險增加,因此抑制它可以降低癌症的風險。重組人EGF是一個6.2 kDa蛋白,含有53個氨基酸殘基。該重組人EGF在53個氨基酸序列之前具有N端His-tag(MW = 8.5 kDa)
Alternate Name | Urogastrone, URG, EGF, epidermal growth factor |
Gene Symbol | EGF |
Gene ID | UniGeneHs.419815 |
Accession # | Q6QBS2 |
Source | E. coli |
Appearance | Recombinant EGF is available as a lyophilized powder |
Physical Form Description | EGF protein is lyophilized with no additives |
Molecular Weight | Human EGF is a 6.2 kDa recombinant protein |
Purity by SDS-PAGE | ≥98% |
Purity by | ≥98% |
Endotoxin Level | <0.1 ng/μg |
Biological Activity | The ED₅₀ of human EGF is determined by the dose-dependent stimulation of BALB/c 3T3 cells is 5.92-10.06 ng/ml. |
Reconstitution Instructions | Reconstitute the human EGF in H₂O to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. |
Handling | Centrifuge the vial prior to opening. |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not to be used in humans |
引用文獻
Wang et al., Evaluation of emulsion electrospun polycaprolactone/hyaluronan/epidermal growth factor nanofibrous scaffolds for wound healing. J Biomater Appl, May 2015; 10.1177/0885328215586907.
An, J. et al. Extensive Determination of Glycan Heterogeneity Reveals an Unusual Abundance of High Mannose Glycans in Enriched Plasma Membranes of Human Embryonic Stem Cells, Mol. Cell. Proteomics, Apr 2012; 11: M111.010660.