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cAMP分析試劑盒 | 貨號K371-100 (abcam ab65355)

cAMP Direct Immunoassay Kit (Colorimetric) 

腺苷3’，5′-環一磷酸酯（環狀AMP，cAMP）是參與許多生理過程的重要“第二信使”。 BioVision的cAMP分析試劑盒可提供直接競爭性免疫分析法，用於靈敏和定量測定生物樣品中的cAMP水平。 該試劑盒利用重組蛋白G包被的96孔板將cAMP多克隆抗體有效地錨定在板上。 cAMP-HRP共軛物與樣品上的cAMP抗體直接競爭，與cAMP競爭。 孵育和洗滌後，可通過讀取OD450 nm處的HRP活性輕鬆確定結合到板上的cAMP-HRP的量。 OD450 nm的強度與樣品中cAMP的濃度成反比。 此外，該試劑盒提供了新的乙酰化步驟，可顯著提高檢測靈敏度。 該試劑盒可檢測〜0.1-10 pmol / 5μl（或〜0.02-2μM）cAMP樣品。  此產品需由儀器進行定量 ELISA儀器設備請按此

Cat # +Size	K371-100
Size	100 assays
Detection Method	Absorbance (450 nm)
Species Reactivity	Mammalian
Applications	The kit can detect ~0.1-10 pmol/5 μl (or ~ 0.02-2 μM) cAMP samples.
Features & Benefits	• Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • The kit provides a new acetylation procedure to improve detection sensitivity significantly
Kit Components	• 10X cAMP Assay Buffer • Standard cAMP (10 nmol) • Neutralizing Buffer • Acetylating Reagent A • Acetylating Reagent B • Rabbit Anti-cAMP pAb • HRP Developer • Protein G Coated Plate
Storage Conditions	-20°C
Shipping Conditions	Gel Pack

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Sun, Yan-Yan et al. (2016) Surface expression of hippocampal NMDA GluN2B receptors regulated by fear conditioning determines its contribution to memory consolidation in adult rats Sci Rep. 2016 Aug 4;6:30743. doi: 10.1038/srep30743.

Li and Lytton. An Essential Role for the K+-dependent Na+/Ca2+-exchanger, NCKX4, in Melanocortin-4-receptor-dependent Satiety. J. Biol. Chem., Sep 2014; 289: 25445 – 25459.

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