Anti-Myc Tag Antibody | 貨號G077

表位標籤(Epitope tags)是短肽序列(short peptide sequences)，易於被標籤特異性抗體識別(tag-specific antibodies)。 由於表位標籤很小，因此不會影響標籤蛋白的生化特性(biochemical properties)。在克隆時，編碼表位標籤的序列最常包含在目標DNA中，以產生包含表位標籤序列(epitope tag sequence)的融合蛋白。 這使得抗表位標籤抗體(anti-epitope tag antibodies)可以用作通用檢測試劑，用於通過重組方法產生的任何包含標籤的蛋白質。 這意味著抗表位標籤抗體是產生特異性抗體以鑑定，免疫沉澱(immunoprecipitate)或免疫親和純化(immunoaffinity purify)重組蛋白的有用替代方法。產生表位標籤融合蛋白的表達載體可用於多種宿主表達系統，包括細菌(bacteria)，酵母(yeast)，昆蟲(insect)和哺乳動物細胞(mammalian cells)。

Product Name	Anti-Myc Tag Antibody (Rabbit Polyclonal)
Description	Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve a universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Subtype	tag
Application	WB, ELISA
Recommended Dilution	ELISA 1:1,000 ; WESTERN BLOT 1:500 – 1:1,000 ; IMMUNOHISTOCHEMISTRY User Optimized ; OTHER APPLICATIONS User Optimized
Raised In	Rabbit
Specificity	This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Immunogen	Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L
Formulation	Liquid
Quantity	100 μg
Clone	Polyclonal
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Concentration	1 mg/ml
Purity	Affinity purified
Isotype	IgG
Species Reactivity	other