人初代胎盤微血管內皮細胞 | Human Primary Placental Microvascular Endothelial Cells 貨號T5449

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Abmgood 正常初代細胞系列商品 (Primary cell)

人初代胎盤微血管內皮細胞 | 貨號 T5449 5×10⁵ cells / 1.0 ml

Human Primary Placental Microvascular Endothelial Cells

初代細胞 (Primary Cell) 是指直接從活體組織中分離出的細胞，具有有限的細胞壽命無法無限制地繼代培養；與一般學術上常用的細胞株 (Cell Line)不同，具有有限的細胞壽命無法無限制地繼代培養，但卻具有最接近活體狀態的細胞型態與功能特性。

Human Primary Placental Microvascular Endothelial Cells細胞Adherent | 來源Placenta (Homo sapiens) | T5449 。細胞Marker: CD31, VE-Cadherin。細胞培養條件: 使用PriCoat™T25培養瓶 (貨號G299)，表面塗有I型膠原組成的細胞外基質，以最佳化初代和不朽細胞的生長、增殖和擴增。或塗層細胞外基質(Applied Cell Extracellular Matrix) 增強細胞貼附 (貨號G422)。Grow cells in ECM-coated culture vessels with the following conditions. 培養液Prigrow X series (貨號TM104)。細胞必須在明膠包被的平板上生長。明膠塗料溶液（0.1％）可以從Abm TM063獲得。細胞只能以1：2的split ratio最多2代。建議每3天進行一次medium更換以保持最佳成長。1%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0°C.

Human Primary Placental Microvascular Endothelial Cells從正常人肝組織中分離出人原代正常胎盤微血管內皮細胞。這些細胞顯示出具有暗核的鵝卵石狀形態。它們表現出CD31和/或VE-鈣黏著蛋白內皮細胞標誌物(VE-Cadherin endothelial cell markers) 的表達。一種有價值的工具，可用於細胞與細胞的粘附測定(cell adhesion assays)，或用於下游過程，例如用於研究應用的RT-PCR或Western blotting。

Organism	Homo sapiens
Source Organ	Placenta
BioSafety Level	II
Growth Properties	Adherent
Morphology	Cobble-stone
Isolation	Isolated from normal human placental tissue
Description	Human Primary Normal Placental Microvascular Endothelial Cells are isolated from normal human liver tissue. The cells exhibit a cobblestone-like morphology with dark nuclei. They exhibit expression of CD31, and/or VE-Cadherin endothelial cell markers. A valuable tool for cell to cell adhesion assays, or for downstream processes such as RT-PCR or Western blotting for research applications.
Applications	For Research Use Only
Markers	CD31, VE-Cadherin
Propagation Requirements	Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM104. Cells must be grown on gelatin coated plates. Gelatin Coating Solution (0.1%) is available at abm, TM063. Cells can only propagate for a maximum of 2 passages at split ratio of 1:2. Recommend to conduct media change every 3rd day to maintain optimal growth. Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

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