磺胺多巴酚B(Sulforhodamine B ,SRB)細胞毒性測定| 貨號K943 (ab235935)

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磺胺多巴酚B(Sulforhodamine B ,SRB)細胞毒性測定法 | 貨號K943 (ab235935)
Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric)
產品介紹: 磺胺多巴酚B(Sulforhodamine B ,SRB)細胞毒性測定法是檢測細胞活力(cell viability)或藥物細胞毒性(drug cytotoxicity)最廣泛的方法之一。該測定法依賴SRB結合細胞蛋白成分並測量總生物量(total biomass)的能力。 SRB是一種亮粉aminoxanthene染料(bright-pink aminoxanthene dye),可以在弱酸性條件下與蛋白質的鹼性氨基酸殘基(basic amino acid residues)形成靜電複合物(electrostatic complex),但在鹼性條件(basic conditions)下可以解離。它已被廣泛用於針對不同類型的癌細胞(cancerous)和非癌細胞株(non-cancerous cell lines)的藥物毒性篩選。另外,該測定法不依賴於細胞代謝活性,因此應顯示較少的受試化合物(testing compounds)干擾。由於SRB的結合是化學計量的,因此洗滌後從染色細胞釋放的摻入染料與細胞生物量成正比,可以在565 nm處測量。 BioVision的SRB細胞細胞毒性測定試劑盒簡單,準確,可重複且靈敏。該試劑盒為體外細胞毒性研究以及高通量藥物篩選提供了出色而有效的方法,每孔可檢測到5,000-50,000個細胞。


| Cat # +Size | K943-1000 (ab235935) |
| Size | 1000 assays |
| Detection Method | Detection method- Absorbance (565 nm) |
| Species Reactivity | Species reactivity- Mammalian |
| Applications | This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of SRB to bind cellular protein components and measure the total biomass. |
| Features & Benefits | • Simple procedure; takes less than 2 hours • Fast, sensitive and convenient |
| Kit Components | • Fixation Solution • 20X Washing Solution • 10X Solubilization Solution • SRB Dye Solution • 20 mM Doxorubicin |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
