中性紅細胞細胞毒性測定 | 貨號K447-1000

Neutral Red Cell Cytotoxicity Assay Kit

產品介紹: 中性紅細胞細胞毒性測定(Neutral red cell cytotoxicity assay)是用於檢測細胞活力(cell viability)或藥物細胞毒性(drug cytotoxicity)的常用方法之一。 該測定的原理是基於通過攝取染料中性紅(neutral red)來檢測活細胞。中性紅是一種真核生物染料(eurhodin dye)，可染色活細胞中的溶酶體(lysosomes)。活細胞可以通過主動轉運吸收中性紅，並將染料摻入其溶酶體(lysosomes)中，但非活細胞不能吸收該生色團(chromophore)。因此，在洗滌後，活細胞可以在酸化提取的條件下釋放摻入的染料。釋放的染料量可用於確定活細胞總數或藥物細胞毒性。中性紅吸收測定法提供了活細胞數量的定量測量，可以在OD 540 nm處測量。 BioVision的中性紅細胞細胞毒性測定試劑盒簡單，準確且可重複。它還包括阿黴素(Doxorubicin)，作為陽性對照(positive control)。該試劑盒為體外細胞毒性研究以及高通量藥物篩選提供了一種出色而有效的方法，每孔可檢測到5,000-50,000個細胞。

Cat # +Size	K447-1000
Size	1000 assays
Kit Summary	• Detection method- Absorbance (540 nm) • Sample type- Cell culture (adherent and non adherent • Species reactivity- Mammalian • Applications- This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of neutral cells to stain lysosomes of viable cells.
Detection Method	Detection method- Absorbance (540 nm)
Species Reactivity	Mammalian
Applications	This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of neutral cells to stain lysosomes of viable cells.
Features & Benefits	• Simple procedure; takes less than 2 hours • Fast, sensitive and convenient
Kit Components	• 100X Neutral Red Staining Solution • 10X Washing Solution • 2X Solubilization Solution • 20 mM Doxorubicin
Storage Conditions	-20°C
Shipping Conditions	Gel Pack