cAMP磷酸二酯酶活性檢測 | cAMP Phosphodiesterase Activity Assay Kit
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特點
產品描述：環腺苷-3’，5′-一磷酸（Cyclic adenosine-3’,5’-monophosphate , cAMP）是真核生物(eukaryotes)中重要的第二信使分子，也是細菌中相關的信號傳導因子(signaling effector)。細胞內cAMP介導的信號級聯通過激活蛋白激酶A(protein kinase A)和環核苷酸門控離子通道(cyclic nucleotide gated ion channels)控制許多生物途徑，例如葡萄糖(glucose)和脂質代謝(lipid metabolism)。細胞內cAMP (Intracellular cAMP) 由ATP腺苷酸環化酶從ATP合成，並被水解的環-AMP磷酸二酯酶（cPDE）滅活。已顯示抑制cPDE影響許多生物學途徑，包括認知(cognition)，神經元信號傳導(neuronal signaling)，炎症(inflammation)和血管平滑肌收縮性(vascular smooth muscle contractility)。 BioVision的cAMP磷酸二酯酶活性檢測試劑盒(cAMP Phosphodiesterase Activity Assay Kit) 提供了一種快速，靈敏，直接的方法來測量各種樣品中的cPDE活性。在該測定中，由cPDE活性產生的AMP被試劑盒組分代謝以產生中間化合物，其與探針反應產生可在Ex / Em = 535 / 587nm處測量的螢光信號。
| Cat # +Size | K2013-100 |
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em = 535/587 nm) |
| Species Reactivity | All |
| Applications | Measurement of Cyclic-AMP Phosphodiesterase activity in various tissues or cells |
| Features & Benefits | • Sensitive • Rapid • Straightforward way to measure cPDE activity in various sample types |
| Kit Components | AMP Standard cPDE Converter cPDE Probe cPDE Assay Buffer cPDE Positive Control cPDE Enzyme Mix cPDE Developer cPDE Substrate |
| Products Name | Cat. No. | Size |
| AMP Colorimetric Assay Kit | K229 | 100 assays |
| cAMP Direct Immunoassay Kit (Colorimetric) | K371 | 100 assays |
| cAMP ELISA Kit | E4715 | 96 assays |
| cAMP Phosphodiesterase Activity Assay Kit (Fluorometric) | K2013 | 100 assays |
| Roflumilast | 2675 | 10 mg, 50 mg |
| (R,S)-Rolipram | 2010 | 5 mg |
| Total Phosphodiesterase Activity Assay Kit (Fluorometric) | K927 | 100 assays |