左旋肉鹼檢測 | L-Carnitine Assay Kit貨號K642-100
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L-Carnitine Assay Kit
產品描述:肉鹼(Carnitine)氨基酸賴氨酸(Lysine)和蛋氨酸(methionine)生物合成的季銨化合物(quaternary ammonium)需要通過肉毒鹼/酰基肉鹼穿梭(carnitine/acylcarnitine shuttle)將脂肪酸(fatty acids)轉運到線粒體基質(mitochondrial matrix)中,其中發生β-氧化(β-oxidation),產生乙酸鹽(acetate)並且在TCA循環中利用乙酸鹽(acetate)產生能量。 左旋肉鹼(L-Carnitine)通常作為營養補充劑出售。 肉鹼以兩種立體異構體(stereoisomers)存在。 只有左旋肉鹼(L-Carnitine)具有生物活性。 BioVision的左旋肉鹼檢測試劑盒(L-Carnitine Assay Kit, k777)是測量生物樣品(如血清)中游離L-肉鹼(free L-Carnitine)的簡便方法。 該測定將乙酰基(acetyl group)從CoA轉移至肉毒鹼(carnitine),並且隨後氧化Oxi-Red探針進一步處理形成的游離CoA,得到螢光(Ex / Em 535nm 587nm)和吸光度(570nm)。 血清L-肉鹼的正常範圍是~20-100μM。 螢光測定法的檢測靈敏度為~1μM,比色測定法的檢測靈敏度為~10μM。
Cat # +Size | K642-100 |
Size | 100 assays |
Detection Method | Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) |
Species Reactivity | Mammalian |
Applications | The detection sensitivity is ~1 µM for the fluorometric assay and ~10 µM for the colorimetric assay. |
Features & Benefits | • Simple procedure; takes ~ 30 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of L-Carnitine levels |
Kit Components | • Carnitine Assay Buffer • Carnitine Probe (in DMSO) • Carnitine Converting Enzyme • Carnitine Substrate Mix • Carnitine Development Mix • Carnitine Standard (10 µmol) |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
引用文獻
Jamie A. et al., Characterization of Pseudomonas aeruginosa Growth on O-Acylcarnitines and Identification of a Short-Chain Acylcarnitine Hydrolase. Appl. Envir. Microbiol., Jun 2013; 79: 3355 – 3363.