TMB Substrate | ELISA Ultrasensitive TMB Substrate for HRP貨號 G444

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相關下載：	ELISA Ultrasensitive TMB Substrate for HRP操作手冊

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ELISA Ultrasensitive TMB Substrate for HRP | 貨號 G444

產品介紹: 3,3’5,5′-Tetramethylbenzidine (TMB) is a safe-sensitive substrate for the detection of horseradish peroxidase (HRP) activity. It yields a blue color (Amax = 652nm) and changes to yellow (Amax = 450nm) upon the addition of either sulfuric or phosphoric acid. ABM’s ELISA Ultrasensitive TMB Substrate for HRP yields the greatest sensitivity among the TMB substrates. 3,3’5,5′-四甲基聯苯胺（,3’5,5′-Tetramethylbenzidine , TMB）是一種安全敏感的底物，用於檢測辣根過氧化物酶（HRP）活性。當加入硫酸或磷酸時，它產生藍色（Amax = 652nm）並變為黃色（Amax = 450nm）。 ABM用於HRP的ELISA超敏感TMB底物在TMB底物中產生最大的靈敏度。

escription	3,3’5,5′-Tetramethylbenzidine (TMB) is a safe-sensitive substrate for the detection of horseradish peroxidase (HRP) activity. It yields a blue colour (Amax = 652nm) and changes to yellow (Amax = 450nm) upon the addition of either sulfuric or phosphoric acid. ABM’s ELISA Ultrasensitive TMB Substrate for HRP yields the greatest sensitivity among the TMB substrates.
Protocol	1. Complete all required incubations with antibodies, probes and HRP labeled reagents. 2. Wash plate, at least 4 times, with phosphate buffered saline or Tris buffered saline containing 0.1% Tween-20. 3. After the final wash, shake and blot all residual buffers from plate wells. 4. Add 0.1ml of ABM’s Ultrasensitive TMB Substrate for HRP to appropriate wells and incubate 5-30 minutes. 5. The reaction can be monitored as a function of time for kinetic assays or stopped with 0.1ml of 0.3mol/l of sulphuric acid or 0.1% sodium fluoride and read at 650nm. 6. If reaction needs to be converted to yellow diimine, add 0.1ml of either sulfuric or phosphoric acid and record the absorbance within 5 minutes.
Application	Suitable for kinetic and endpoint assays involving Horseradish Peroxidase (HRP).
Storage	Store at room temperature (15-28°C) or at 2-4°C and then warm up to assay temperature prior to use. Prevent exposure to direct sunlight. Discard if solution turns blue or turbid.

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