Taq DNA Ligase | 貨號 E090
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產品介紹: Taq DNA連接酶(Taq DNA Ligase)是熱穩定連接酶(thermostable ligase)，其催化雙鏈DNA的相鄰5′-磷酸和3′-羥基(3’-hydroxyl groups)之間的磷酸二酯鍵的形成。 該酶是NAD +依賴性DNA連接酶(NAD+ dependent DNA ligase)。一個單位定義為在45℃下15分鐘內在50μl的總反應體積中1μg BstEII消化的DNA的12bp粘性末端50％連接的量。
Nick-selective ligation activity means there is no end-joining of DNA fragments.
High-fidelity ligation – no ligation of nicks containing base pair mismatches.
SNP detection using Ligase Chain Reaction and Ligase Detection Reaction.
Mutagenesis by incorporation of a mutagenic oligonucletide that has been phosphorylated.
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| Description | Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between adjacent 5’-phosphate and 3’-hydroxyl groups of duplex DNA. This enzyme is a NAD+ dependent DNA ligase. |
| Application |
· Nick-selective ligation activity means there is no end-joining of DNA fragments. · High-fidelity ligation – no ligation of nicks containing base pair mismatches. · SNP detection using Ligase Chain Reaction and Ligase Detection Reaction. · Mutagenesis by incorporation of a mutagenic oligonucletide that has been phosphorylated. |
| Form | Enzyme supplied with a 10X Reaction Buffer. Incubate at 45°C. |
| Concentration | 40000 U/ml |
| Storage | Store all components at -20°C upon arrival. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. For long term storage, store the enzyme at -20°C, and the reaction buffer at -80°C to extend the half life of NAD+. |
| Storage Buffer | 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol. |
| Notes |
One unit is defined as the amount of enzyme required to give 50% ligation of the 12 bp cohesive ends of 1 µg of BstEII-digested λ DNA in a total reaction volume of 50 µl in 15 min at 45°C. Taq DNA Ligase cannot be used as a substitute for T4 DNA Ligase. This product is distributed for laboratory research only. |