GFP Tag多株抗體 |polyclonal anti-GFP antibody 貨號G095
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特點
產品介紹: GFP Tag多株抗體，免疫原為免疫原是綠色螢光蛋白（Green Fluorescent Protein , GFP）融合蛋白，來自維多利亞水母（Aequorea victoria）的全長氨基酸序列（246aa）。此抗體通過免疫親和層析，使用與瓊脂糖珠偶聯的綠色螢光蛋白（Aequorea victoria），然後進行固相吸附，從單特異性抗血清製備該產物，以除去任何不需要的反應性。抗體純化後，保存於PBS, pH 7.4 with 0.05% sodium azide。抗體濃度為100µg/100ul，最佳實驗濃度請參考操作手冊。
抗體之保存: 避免反覆冷凍解凍；4°C保存；若需長期保存請貯存於-20°C。可應用於WB, IHC, IP, ELISA, IF實驗。
| Product Name | Anti-GFP Tag Antibody (Goat Polyclonal) |
| Subtype | tag |
| Application | WB, IHC, IP, ELISA, IF |
| Recommended Dilution | ELISA 1:40,000; WESTERN BLOT 1:400 – 1:2,000; IMMUNOHISTOCHEMISTRY 1:200 – 1:1,000; IF MICROSCOPY 1:1,000; IP 1:200 |
| Raised In | goat |
| Specificity | Recognize GFP and its variants. |
| Immunogen | The anti-GFP immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
| Formulation | Liquid |
| Quantity | 100 μg |
| Clone | N/A |
| Storage | This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Storage Buffer | PBS, pH 7.4 with 0.05% sodium azide. |
| Concentration | 100µg/100ul |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins. |
| Isotype | IgG |
| Species Reactivity | other |
引用文獻
Sutton, LM et al. “Hip14l-deficient mice develop neuropathological and behavioural features of Huntington disease.” Hum Mol Genet 22 (3):452-465 (2013). DOI: 10.1093/hmg/dds441. Application: Immunoprecipitation.
Luchniak, A et al. “Structure-function analysis of yeast.” Methods Cell Biol 115:355-374 (2013). DOI: 10.1016/B978-0-12-407757-7.00022-0. Application: SDS-PAGE.