葡萄糖激酶活性測定套組 | 貨號K969-100

PicoProbe™ Glucokinase Activity Assay Kit (Fluorometric)

產品描述：Glucokinase葡萄糖激酶（也稱為GCK，己糖激酶IV型或D和ATP：D-己糖6-磷酸轉移酶; EC 2.7.1.1）在特定類型的組織中表達：肝臟(liver)，胰腺(pancreas)，小腸(small intestine)和腦(brain)。葡萄糖激酶(Glucokinase)作為葡萄糖傳感器(glucose sensor)起作用，反應於血液中葡萄糖的水平，例如營養(nutritional)和激素分子途徑(hormonal molecular pathways)，引發碳水化合物代謝(hyperglycemia)或細胞功能的變化。與其他己糖激酶(Hexokinases)不同，葡萄糖激酶對葡萄糖的親和力相對較低，並且不受生理濃度的葡萄糖-6-磷酸的抑制。gene encoding GCK的基因突變可引起高血糖症(hyperglycemia)和低血糖症(hypoglycemia)。由於葡萄糖激酶(Glucokinase)在控制血糖穩態中具主要作用，葡萄糖激酶(Glucokinase)目前被認為是治療高血糖症強有力候選靶標，高血糖症是2型糖尿病(Type 2 Diabetic patients)患者中遇到的病症。BioVision的PicoProbe™葡萄糖激酶活性測定試劑盒 (PicoProbe™ Glucokinase Activity Assay Kit, K816-6-100)。為各種樣品中的GCK活性監測，提供了一種快速簡便的方法。在該測定中，GCK將葡萄糖轉化為葡萄糖-6-磷酸(glucose-6-phosphate)，其又轉化為一系列中間體，其減少PicoProbe ^TM，產生強螢光產物（Ex / Em = 535 / 587nm）。該測定法簡單，特異，靈敏且適應高通量，可檢測低至2μU的GCK活性。

Cat # +Size	K969-100
Size	100 assays
Kit Summary	• Detection method- Fluorescence (Ex/Em = 535/587 nm) • Applications-Measurement of Glucokinase activity in various tissues/cells. • Analysis of Glucose metabolism and Cell signaling in various cell types. • Screening anti-diabetic drugs.
Detection Method	Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity	Eukaryotes
Applications	• Measurement of Glucokinase activity in various tissues/cells. • Analysis of Glucose metabolism and Cell signaling in various cell types. • Screening anti-diabetic drugs.
Features & Benefits	• Simple and rapid procedure • Highly Sensitive • Can detect glucokinase activity even as low as 2 µU
Kit Components	• GCK Assay Buffer • PicoProbe™ (in DMSO) • DTT (1 M) • GCK Substrate • Sample Background Reagent • ATP • GCK Enzyme Mix • GCK Developer • GCK Positive Control • NAPDH Standard (200 nmol)