高靈敏度Malachite green磷酸根呈色法 |貨號303-0125(ABCAM ab270004)

PiColorLock Gold Phosphate Detection System

產品介紹: PiColorLock™磷酸根檢測試劑，為檢測無機磷酸根（Pi）之含量；使用Malachite green磷酸根呈色法。可用於測量產生Pi的任何酶，包括ATPases、GTPases, phosphatases、熱休克蛋白及DNA解旋蛋白。PiColorLock™的獨特配方提高了測定線性，動態範圍和顏色穩定性；也可以用PiColorLock與不穩定的底物（例如ATP，GTP）一起使用，所述底物與其它酸性染料基檢測試劑產生高的非酶背景信號。此產品不具放射性應用Malachite green磷酸根呈色法，為高靈敏的磷酸根偵測試劑，確保穩定的綠色dye-phosphate複合物產生，避免發生受質非酵素水解干擾造成的背景雜訊。偵測範圍廣，可減少樣品稀釋的必要。

Key benefits:

• Colorimetric assay – Simple, scalable and nonradioactive
• Compatible with almost any assay buffer – Flexibility over a wide range of existing assays
• Stable reagent formulation – Long shelf life
• Unique accelerator – Speeds up color development
• Unique stabilizer – Suppresses nonenzymatic backgrounds with acid labile substrates (ATP or GTP)
• PiColorLock™-Pi complex is very stable – No precipitation, results can be measured over several hours; ideal for HTS
• Wide linear range – No inhibition of color development by high concentrations of protein
• The unique PiColorLock™ Stabilizer prevents background drift with time.

 

Figure 1. Competitor assays are beset with several problems including reagent precipitation. The high stability of PiColorLock™ ensures high stability of the colored dye–phosphate complexes (green color).

 

Figure 2. ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ provides baseline readings.

 

Figure 3. PiColorLock™ has also been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.

 

產品型號	產品名稱	包裝
303-0125 303-0500	PiColorLock Gold Phosphate Detection System（靈敏度4－40uM）	最多1560 assays 最多6250 assays
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