Streptavidin-UNLB | 貨號7100-01  規格1ml  (5.0 mg/mL) (貨號異動為7105-01)

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

產品介紹:  Streptavidin-UNLB。針對Biotin具特異性。來源為Streptomyces avidinii。 緩衝液配方在Borate buffered saline, pH 8.2；濃度為5.0 mg/mL。1.0 mL

Source	Streptomyces avidinii
Specificity	Biotin
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2
Concentration	5.0 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	ELISA – Quality tested 1-7 FLISA – Quality tested Flow Cytometry – Quality tested 1,2,4,8-15 ELISpot – Reported in literature 4,6-8 Immunohistochemistry-Frozen Sections – Reported in literature 9,10,13,16-19 Immunohistochemistry-Paraffin Sections – Reported in literature 16,18,21 Immunocytochemistry – Reported in literature 11,17,19 Western Blot – Reported in literature 3,5,20 Multiplex – Reported in literature 22 Microarray – Reported in literature 23 Blocking – Reported in literature 24

Frozen chicken intestine tissue section was stained with Mouse Anti-Chicken IgA-BIOT (SB Cat. No. 8330-08) followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.

Cat. No.	Product Description	Size
17100-01	Rat Anti-Human Caspase-2-UNLB	0.1 mg
7100-04	Streptavidin-AP	1.0 mL
7100-11L	Streptavidin-APC	1.0 mg
7100-11M	Streptavidin-APC	0.5 mg
7100-11S	Streptavidin-APC	0.1 mg
7100-19L	Streptavidin-APC/CY7	0.5 mg
7100-19S	Streptavidin-APC/CY7	0.1 mg
7100-06	Streptavidin-BGAL	1.0 mL
7100-21	Streptavidin-CY2	1.0 mg
7100-12	Streptavidin-CY3	0.1 mg
7100-24	Streptavidin-CY3.5	0.1 mg
7100-15	Streptavidin-CY5	1.0 mg
7100-02	Streptavidin-FITC	1.0 mg
7100-02S	Streptavidin-FITC	0.5 mg
7100-05	Streptavidin-HRP	1.0 mL

References

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2. Gulbranson-Judge A, Tybulewicz VL, Walters AE, Toellner K, MacLennan IC, Turner M. Defective immunoglobulin class switching in Vav-deficient mice is attributable to compromised T cell help. Eur J Immunol. 1999;29:477-87. (ELISA, FC)
3. Utani A, Nomizu M, Matsuura H, Kato K, Kobayashi T, Takeda U, et al. A unique sequence of the laminin α3 G domain binds to heparin and promotes cell adhesion through syndecan-2 and -4. J Biol Chem. 2001;276:28779-88. (ELISA, WB)
4. Taylor DK, Ito E, Thorn M, Sundar K, Tedder T, Spatz LA. Loss of tolerance of anti-dsDNA B cells in mice overexpressing CD19. Mol Immunol. 2006;43:1776-90. (ELISA, ELISPOT, FC)
5. Doern A, Cao X, Sereno A, Reyes CL, Altshuler A, Huang F, et al. Characterization of inhibitory anti-insulin-like growth factor receptor antibodies with different epitope specificity and ligand-blocking properties: implications for mechanism of action in vivo. J Biol Chem. 2009;284:10254-67. (ELISA, WB)
6. Vandenberg CJ, Josefsson EC, Campbell KJ, James C, Lawlor KE, Kile BT, et al. Loss of Bak enhances lymphocytosis but does not ameliorate thrombocytopaenia in BCL-2 transgenic mice. Cell Death Differ. 2014;21:676-84. (ELISA, ELISPOT)
7. Dunand CJ, Leon PE, Kaur K, Tan GS, Zheng N, Andrews S, et al. Preexisting human antibodies neutralize recently emerged H7N9 influenza strains. J Clin Invest. 2015;125:1255-68. (ELISA, ELISPOT)
8. Ju ZL, Shi GY, Zuo JX, Zhang JW, Sun J. Unexpected development of autoimmunity in BAFF-R-mutant MRL-lpr mice. Immunology. 2006;120:281-9. (ELISPOT, FC)
9. Kapasi ZF, Qin D, Kerr WG, Kosco-Vilbois MH, Shultz LD, Tew JG, et al. Follicular dendritic cell (FDC) precursors in primary lymphoid tissues. J Immunol. 1998;160:1078-84. (FC, IHC-FS)
10. Brakebusch C, Fillatreau S, Potocnik AJ, Bungartz G, Wilhelm P, Svensson M, et al. β1 integrin is not essential for hematopoiesis but is necessary for the T cell-dependent IgM antibody response. Immunity. 2002;16:465-77. (FC, IHC-FS)
11. Moura IC, Arcos-Fajardo M, Sadaka C, Leroy V, Benhamou M, Novak J, et al. Glycosylation and size of IgA1 are essential for interaction with mesangial transferrin receptor in IgA nephropathy. J Am Soc Nephrol. 2004;15:622-34. (FC, ICC)
12. Tsuneto M, Yamazaki H, Yoshino M, Yamada T, Hayashi S. Ascorbic acid promotes osteoclastogenesis from embryonic stem cells. Biochem Biophys Res Commun. 2005;335:1239-46. (FC, Sep)
13. Zotos D, Coquet JM, Zhang Y, Light A, D’Costa K, Kallies A, et al. IL-21 regulates germinal center B cell differentiation and proliferation through a B cell-intrinsic mechanism. J Exp Med. 2010;207:365-78. (FC, IHC-FS)
14. Thorum SC, Comstock SS, Hester SN, Shunk JM, Monaco MH, Donovan SM. Impact of diet on development of bronchial-associated immunity in the neonatal piglet. Vet Immunol Immunopathol. 2013;151:63-72. (FC)
15. Jung S, Schickel J, Kern A, Knapp A, Eftekhari P, Da Silva S, et al. Chronic bacterial infection activates autoreactive B cells and induces isotype switching and autoantigen-driven mutations. Eur J Immunol. 2016;46:131-46. (FC)
16. Nosanchuk JD, Valadon P, Feldmesser M, Casadevall A. Melanization of Cryptococcus neoformans in murine infection. Mol Cell Biol. 1999;19:745-50. (IHC-FS, IHC-PS)
17. Zannettino AC, Holding CA, Diamond P, Atkins GJ, Kostakis P, Farrugia A, et al. Osteoprotegerin (OPG) is localized to the Weibel-Palade bodies of human vascular endothelial cells and is physically associated with von Willebrand factor. J Cell Physiol. 2005;204:714-23. (IHC-FS, ICC)
18. Ji F, Liu Z, Cao J, Li N, Liu Z, Zuo J, et al. B cell response is required for granuloma formation in the early infection of Schistosoma japonicum. PLoS One. 2008;3(3):e1724. (IHC-FS, IHC-PS)
19. Piersma B, de Rond S, Werker PM, Boo S, Hinz B, van Beuge MM, et al. YAP1 is a driver of myofibroblast differentiation in normal and diseased fibroblasts. Am J Pathol. 2015;185:3326-37. (IHC-FS, ICC)
20. Ren B, Li J, Genschmer K, Hollingshead SK, Briles DE. The absence of PspA or presence of antibody to PspA facilitates the complement-dependent phagocytosis of pneumococci in vitro. Clin Vaccine Immunol. 2012;19:1574-82. (WB)
21. El Karoui K, Viau A, Dellis O, Bagattin A, Nguyen C, Baron W, et al. Endoplasmic reticulum stress drives proteinuria-induced kidney lesions via Lipocalin 2. Nat Commun. 2016;7:10330. (IHC-PS)
22. Piccioli D, Tavarini S, Borgogni E, Steri V, Nuti S, Sammicheli C, et al. Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets. Blood. 2007;109:5371-9. (Multiplex)
23. Timmerman P, Barderas R, Desmet J, Altschuh D, Shochat S, Hollestelle MJ, et al. A combinatorial approach for the design of complementarity-determining region-derived peptidomimetics with in vitro anti-tumoral activity. J Biol Chem. 2009;284:34126-34. (Microarray)
24. Irie-Maezono R, Tsuyama S. Immunohistochemical analysis of the acid secretion potency in gastric parietal cells. CellBio. 2013;2:179-85. (Block)