Mouse Anti-Chicken CD8α-PE抗體 | 貨號8220-09   規格1.0 ml  (1.0 mg/mL)

產品介紹:  在雞中，CD8分子以兩種形式存在 – （i）兩條α鏈的同型二聚體(homodimer) 和（ii）α鍊和β鏈的異二聚體 (heterodimer) 。 雖然成年雞的胸腺(thymus) ，脾臟(spleen) 和血液(blood)中的絕大多數CD8 +細胞均表達CD8α-和CD8β-鏈，但胚胎和幼雛脾臟中相對大比例的CD8 +TCRγδ細胞僅表達α-鏈。 CD8 在腸上皮淋巴細胞中，小鼠中存在的主要CD8 + T細胞群具保守，但是在囓齒動物中沒有發現雞中的TCRγδCD8αβ細胞群。 雞CD8在大約80％的胸腺細胞 (thymocytes) ，15％的血液單核細胞(blood mononuclear cells) 和50％的脾細胞（spleen cells）　中表達，但在法氏囊和骨髓中的表達不到1％。 單克隆抗體CT-8識別CD8α鏈，並且已顯示出與火雞中的多態性決定簇反應（polymorphic determinant）。

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD8α-PE (SB Cat. No. 8220-09) and Mouse Anti-Chicken CD4-FITC (SB Cat. No. 8210-02).

Clone	CT-8
Isotype	Mouse (BALB/c) IgG1κ
Isotype Control	Mouse IgG1-PE
Immunogen	Chicken thymocytes and Ig-negative blood leukocytes
Specificity	Chicken/Turkey/Guinea Fowl/Pigeon CD8α
Description	In the chicken, the CD8 molecule is present in two forms – (i) a homodimer of two α chains and (ii) a heterodimer of an α chain and a β chain. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8α- and CD8β-chains, a relatively large proportion of the CD8+ TCRγδ cells in the spleens of embryos and young chicks express only the α-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRγδ CD8αβ cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody CT-8 recognizes the CD8α chain and has been shown to react to a polymorphic determinant in turkey.
Format/Conjugate	PE (R-phycoerythrin)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration	0.1 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light; Do not freeze Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – Flow Cytometry – Quality tested 1,9-16,18 Immunohistochemistry-Frozen Sections – Reported in literature 2-5 Immunohistochemistry-Paraffin Sections – Reported in literature 6-8 Immunocytochemistry – Reported in literature 17 Immunoprecipitation – Reported in literature 1,17 Blocking – Reported in literature 1