Mouse Anti-Chicken CD8β-PE抗體 | 貨號8280-09  規格1.0 mL  (0.1 mg/mL)

產品介紹: 在雞中，CD8分子以兩種形式存在 – （i）兩條α鏈的同型二聚體 (homodimer) 和（ii）α鍊和β鏈的異二聚體 (heterodimer)。 雖然成年雞的胸腺(thymus)、脾臟(spleen)、和血液(blood)中的絕大多數CD8 +細胞均表達CD8α-和CD8β-鏈，但胚胎(embryos)和幼雛脾臟中相對大比例的CD8 + TCR-γδ細胞僅表達α  –  CD8的鏈。 在腸上皮淋巴細胞 (intestinal epithelial lymphocytes)中，小鼠中存在的主要CD8 + T細胞群是保守 (conserved) 的，但在雞中存在TCR-γδCD8αβ細胞群，其在囓齒動物 (rodents) 中未發現。 雞CD8在大約80％的胸腺細胞 (thymocytes)，45％的血液單核細胞 (blood mononuclear cells) 和50％的脾細胞 (spleen cells) 中表達，但在法氏囊(bursa) 和骨髓 (bone marrow) 中的表達不到1％。 單株抗體EP42識別CD8β鏈。Mouse Anti-Chicken CD8β-PE抗體，具PE螢光標誌。免疫原為Chicken splenocytes針對Chicken CD8β具特異性。Isotype為Mouse (BALB/c) IgG_2aκ。Isotype Control Mouse IgG_2a-PE。緩衝液配方在Phosphate buffered saline 含有 0.1% sodium azide防腐劑及 stabilizer。

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD8β-PE (SB Cat. No. 8280-09) and Mouse Anti-Chicken CD4-FITC (SB Cat. No. 8210-02)

Clone	EP42
Isotype	Mouse (BALB/c) IgG2aκ
Isotype Control	Mouse IgG2a-PE
Immunogen	Chicken splenocytes
Specificity	Chicken CD8β
Description	In the chicken, the CD8 molecule is present in two forms – (i) a homodimer of two α chains and (ii) a heterodimer of an α chain and a β chain. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8α- and CD8β-chains, a relatively large proportion of the CD8+ TCR-γδ cells in the spleens of embryos and young chicks express only the α-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCR-γδ CD8αβ cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody EP42 recognizes the CD8β chain.
Format/Conjugate	PE (R-phycoerythrin)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration	0.1 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light; Do not freeze Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – Flow Cytometry – Quality tested 1,2,4-9 Immunohistochemistry-Frozen Sections – Reported in literature 2,3 Immunoprecipitation – Reported in literature 1
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796481

引用文獻References

1. Tregaskes CA, Kong F, Paramithiotis E, Chen CH, Ratcliffe MJ, Davison TF, et al. Identification and analysis of the expression of CD8αβ and CD8αα isoforms in chickens reveals a major TCR-γδ CD8αβ subset of intestinal intraepithelial lymphocytes. J Immunol. 1995;154:4485-94. (Immunogen, FC, IP)
2. van Haarlam DA, van Kooten PJ, Rothwell L, Kaiser P, Vervelde L. Characterisation and expression analysis of the chicken interleukin-7 receptor alpha chain. Dev Comp Immunol. 2009;33:1018-26. (FC, IHC-FS)
3. Pielsticker C. Investigations on the humoral and cell-mediated immune response in chicken after inoculation with Campylobacter jejuni strains of human and avian origin [thesis]. Hannover (Germany): University of Veterinary Medicine Hannover; 2013. (IHC-FS)
4. Buitenhuis AJ, Kjaer JB, Labouriau R, Juul-Madsen HR. Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior. Poult Sci. 2006;85:1722-8. (FC)
5. van Ginkel FW, van Santen VL, Gulley SL, Toro H. Infectious bronchitis virus in the chicken Harderian gland and lachrymal fluid: viral load, infectivity, immune cell responses, and effects of viral immunodeficiency. Avian Dis. 2008;52:608-17. (FC)
6. Dalgaard T, Boving MK, Handberg K, Jensen KH, Norup LR, Juul-Madsen HR. MHC expression on spleen lymphocyte subsets in genetically resistant and susceptible chickens infected with Marek’s disease virus. Viral Immunol. 2009;22:321-7. (FC)
7. Norup LR, Dalgaard TS, Friggens NC, Sørensen P, Juul-Madsen HR. Influence of chicken serum mannose-binding lectin levels on the immune response towards Escherichia coli. Poult Sci. 2009;88:543-53. (FC)
8. Petrov P, Motobu M, Salmi J, Uchida T, Vainio O. Novel leukocyte protein, Trojan, differentially expressed during thymocyte development. Mol Immunol. 2010;47:1522-8. (FC)
9. Mwangi WN, Smith LP, Baigent SJ, Beal RK, Nair V, Smith AL. Clonal structure of rapid-onset MDV-driven CD4+ lymphomas and responding CD8+ T cells. PLoS Pathog. 2011;7(5):e1001337. (FC)

Cat. No.	Product Description	Clone	Size
8280-08	Mouse Anti-Chicken CD8β-BIOT	EP42	0.5 mg
8280-02	Mouse Anti-Chicken CD8β-FITC	EP42	0.5 mg
8280-09	Mouse Anti-Chicken CD8β-PE	EP42	0.1 mg
8280-01	Mouse Anti-Chicken CD8β-UNLB	EP42	0.5 mg