Mouse Anti-Chicken CD8β-PE抗體 | Southernbiotech SBA貨號8280-09

代理廠牌：
原廠連結：	https://www.southernbiotech.com
相關下載：	Mouse Mouse Anti-Chicken CD8β-PE抗體操作手冊

特點

Mouse Anti-Chicken CD8β-PE抗體 | 貨號8280-09 規格1.0 mL (0.1 mg/mL)

產品介紹: 在雞中，CD8分子以兩種形式存在 – （i）兩條α鏈的同型二聚體 (homodimer) 和（ii）α鍊和β鏈的異二聚體 (heterodimer)。雖然成年雞的胸腺(thymus)、脾臟(spleen)、和血液(blood)中的絕大多數CD8 +細胞均表達CD8α-和CD8β-鏈，但胚胎(embryos)和幼雛脾臟中相對大比例的CD8 + TCR-γδ細胞僅表達α – CD8的鏈。在腸上皮淋巴細胞 (intestinal epithelial lymphocytes)中，小鼠中存在的主要CD8 + T細胞群是保守 (conserved) 的，但在雞中存在TCR-γδCD8αβ細胞群，其在囓齒動物 (rodents) 中未發現。雞CD8在大約80％的胸腺細胞 (thymocytes)，45％的血液單核細胞 (blood mononuclear cells) 和50％的脾細胞 (spleen cells) 中表達，但在法氏囊(bursa) 和骨髓 (bone marrow) 中的表達不到1％。單株抗體EP42識別CD8β鏈。Mouse Anti-Chicken CD8β-PE抗體，具PE螢光標誌。免疫原為Chicken splenocytes針對Chicken CD8β具特異性。Isotype為Mouse (BALB/c) IgG_2aκ。Isotype Control Mouse IgG_2a-PE。緩衝液配方在Phosphate buffered saline 含有 0.1% sodium azide防腐劑及 stabilizer。

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD8β-PE (SB Cat. No. 8280-09) and Mouse Anti-Chicken CD4-FITC (SB Cat. No. 8210-02)

Clone	EP42
Isotype	Mouse (BALB/c) IgG_2aκ
Isotype Control	Mouse IgG_2a-PE
Immunogen	Chicken splenocytes
Specificity	Chicken CD8β
Description	In the chicken, the CD8 molecule is present in two forms – (i) a homodimer of two α chains and (ii) a heterodimer of an α chain and a β chain. While the vast majority of CD8⁺ cells in the thymus, spleen, and blood of adult chickens express both CD8α- and CD8β-chains, a relatively large proportion of the CD8⁺ TCR-γδ cells in the spleens of embryos and young chicks express only the α-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8⁺ T cell populations present in mice are conserved but there is a population of TCR-γδ CD8αβ cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody EP42 recognizes the CD8β chain.
Format/Conjugate	PE (R-phycoerythrin)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration	0.1 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light; Do not freeze Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – Flow Cytometry – Quality tested ^1,2,4-9 Immunohistochemistry-Frozen Sections – Reported in literature ^2,3 Immunoprecipitation – Reported in literature ¹
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796481

引用文獻References

Tregaskes CA, Kong F, Paramithiotis E, Chen CH, Ratcliffe MJ, Davison TF, et al. Identification and analysis of the expression of CD8αβ and CD8αα isoforms in chickens reveals a major TCR-γδ CD8αβ subset of intestinal intraepithelial lymphocytes. J Immunol. 1995;154:4485-94. (Immunogen, FC, IP)
van Haarlam DA, van Kooten PJ, Rothwell L, Kaiser P, Vervelde L. Characterisation and expression analysis of the chicken interleukin-7 receptor alpha chain. Dev Comp Immunol. 2009;33:1018-26. (FC, IHC-FS)
Pielsticker C. Investigations on the humoral and cell-mediated immune response in chicken after inoculation with Campylobacter jejuni strains of human and avian origin [thesis]. Hannover (Germany): University of Veterinary Medicine Hannover; 2013. (IHC-FS)
Buitenhuis AJ, Kjaer JB, Labouriau R, Juul-Madsen HR. Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior. Poult Sci. 2006;85:1722-8. (FC)
van Ginkel FW, van Santen VL, Gulley SL, Toro H. Infectious bronchitis virus in the chicken Harderian gland and lachrymal fluid: viral load, infectivity, immune cell responses, and effects of viral immunodeficiency. Avian Dis. 2008;52:608-17. (FC)
Dalgaard T, Boving MK, Handberg K, Jensen KH, Norup LR, Juul-Madsen HR. MHC expression on spleen lymphocyte subsets in genetically resistant and susceptible chickens infected with Marek’s disease virus. Viral Immunol. 2009;22:321-7. (FC)
Norup LR, Dalgaard TS, Friggens NC, Sørensen P, Juul-Madsen HR. Influence of chicken serum mannose-binding lectin levels on the immune response towards Escherichia coli. Poult Sci. 2009;88:543-53. (FC)
Petrov P, Motobu M, Salmi J, Uchida T, Vainio O. Novel leukocyte protein, Trojan, differentially expressed during thymocyte development. Mol Immunol. 2010;47:1522-8. (FC)
Mwangi WN, Smith LP, Baigent SJ, Beal RK, Nair V, Smith AL. Clonal structure of rapid-onset MDV-driven CD4+ lymphomas and responding CD8+ T cells. PLoS Pathog. 2011;7(5):e1001337. (FC)

Cat. No.	Product Description	Clone	Size
8280-08	Mouse Anti-Chicken CD8β-BIOT	EP42	0.5 mg
8280-02	Mouse Anti-Chicken CD8β-FITC	EP42	0.5 mg
8280-09	Mouse Anti-Chicken CD8β-PE	EP42	0.1 mg
8280-01	Mouse Anti-Chicken CD8β-UNLB	EP42	0.5 mg

↑回到頁首↑