NADP / NADPH定量試劑盒 | 貨號K347-100 (ABCAM ab65349)

NADP/NADPH Quantitation Colorimetric Kit

產品描述：煙酰胺核苷酸 (nicotinamide nucleotides) 的測定在細胞或組織的能量轉化和氧化還原狀態的研究中持續受到關注。 NADP / NADPH定量試劑盒為細胞內核苷酸的靈敏檢測提供了便利的工具：NADP，NADPH及其比例。 系統中的酶特異性識別酶循環反應中的NADP / NADPH（它不識別NAD + / NADH）。 無需從樣品混合物中純化NADP / NADPH。 酶循環反應顯著提高了檢測靈敏度。 可以使用plate reader在OD450nm下定量。

Cat # +Size	K347-100 (ABCAM ab65349)
Size	100 assays
Detection Method	Absorbance (450 nm)
Species Reactivity	Mammalian
Applications	Convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio
Features & Benefits	• Simple procedure; takes ~2 hours • Fast and convenient • Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio • Enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH)
Kit Components	• NADP/NADPH Extraction Buffer • NADP Cycling Buffer • NADP Cycling Enzyme Mix • NADPH Developer • Stop Solution • NADPH Standard (MW:833.36)
Storage Conditions	-20°C

引用文獻

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Chaman et al., ERK2-Pyruvate Kinase Axis Permits Phorbol 12-Myristate 13-Acetate-induced Megakaryocyte Differentiation in K562 Cells.  J. Biol. Chem., Sep 2015; 290: 23803 – 23815.

Zhao et al., Regenerative Neurogenesis After Ischemic Stroke Promoted by Nicotinamide Phosphoribosyltransferase–Nicotinamide Adenine Dinucleotide Cascade. Stroke, Jul 2015; 46: 1966 – 1974.

Li et al., Mitofusin-2-mediated tethering of mitochondria and endoplasmic reticulum promotes cell cycle arrest of vascular smooth muscle cells in G0/G1 phase. Acta Biochim Biophys Sin, Jun 2015; 47: 441 – 450.