Mouse IgG1-UNLB | 貨號0102-01

產品介紹:  Mouse IgG1-UNLB，不具標誌。針對T-2 mycotoxin具特異性。Isotype為Mouse IgG₁κ。緩衝液配方在Borate buffered saline, pH 8.2。

Frozen newborn mouse cartilage section was stained with Mouse IgG1-UNLB isotype control (SB Cat. No. 0102-01; left) and Mouse Anti-Type II Collagen-UNLB (right) followed by Goat Anti-Mouse IgG1, Human ads-FITC (SB Cat. No. 1070-02) and DAPI.

ELISA plate was coated with serially diluted Mouse IgG1-UNLB (SB Cat. No. 0102-01). Immunoglobulin was detected with Goat Anti-Mouse IgG1, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08), Goat Anti-Mouse IgG2b, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG3, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.

Paraffin embedded human gastric cancer tissue was stained with Mouse IgG1-UNLB isotype control (SB Cat. No. 0102-01; left) and Mouse Anti-Human MMP-2-UNLB (SB Cat. No. 12015-01; right) followed by HRP conjugated Anti-Mouse Ig secondary antibody, DAB, and hematoxylin.

Clone	15H6
Isotype	Mouse IgG1κ
Specificity	T-2 mycotoxin
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2
Concentration	1.0 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – Flow Cytometry – Quality tested 5-10,12,14 ELISA – Quality tested 1-4 FLISA – Quality tested Immunohistochemistry-Frozen Sections – Reported in literature 11,12 Immunohistochemistry-Paraffin Sections – Reported in literature 13,23 Immunocytochemistry – Reported in literature 14,15 Western Blot – Reported in literature 16,17 Blocking – Reported in literature 8 In vitro Control – Reported in literature 5,7,12,14,18 In vivo Control – Reported in literature 19-21 Multiplex – Reported in literature 24
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2793845

References文獻資料

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2. Faustino L, Fonseca DM, Florsheim EB, Resende RR, Lepique AP, Faquim-Mauro E, et al. Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation. Mucosal Immunol. 2014;7:1199-208. (ELISA)
3. DiLillo DJ, Tan GS, Palese P, Ravetch JV. Broadly neutralizing hemagglutinin stalk-specific antibodies require FcγR interactions for protection against influenza virus in vivo. Nat Med. 2014;20:143-51. (ELISA)
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7. Chua W, Kim S, Myers N, Huang S, Yu L, Fremont DH, et al. Endogenous MHC-related protein 1 is transiently expressed on the plasma membrane in a conformation that activates mucosal-associated invariant T cells. J Immunol. 2011;186:4744-50. (FC, in vitro control)
8. Sinka L, Biasch K, Khazaal I, Péault B, Tavian M. Angiotensin-converting enzyme (CD143) specifies emerging lympho-hematopoietic progenitors in the human embryo. Blood. 2012;119:3712-23. (FC, Block)
9. Troyer RM, Thompson J, Elder JH, VandeWoude S. Accessory genes confer a high replication rate to virulent feline immunodeficiency virus. J Virol. 2013;87:7940-51. (FC)
10. Weckbach LT, Gola A, Winkelmann M, Jakob SM, Groesser L, Borgolte J, et al. The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via β2 integrins (CD11/CD18). Blood. 2014;123:1887-96. (FC)
11. Kiekens RC, Thepen T, Bihari IC, Knol EF, Van De Winkel JG, Bruijnzeel-Koomen CA. Expression of Fc receptors for IgG during acute and chronic cutaneous inflammation in atopic dermatitis. Br J Dermatol. 2000;142:1106-13. (IHC-FS)
12. Cosmi L, Liotta F, Lazzeri E, Francalanci M, Angeli R, Mazzinghi B, et al. Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. Blood. 2003;102:4107-14. (FC, IHC-FS, in vitro control)
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14. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (FC, ICC, in vitro control)
15. Sleeman KE, Kendrick H, Ashworth A, Isacke CM, Smalley MJ. CD24 staining of mouse mammary gland cells defines luminal epithelial, myoepithelial/basal and non-epithelial cells. Breast Cancer Res. 2006;8:R7. (ICC)
16. Howell MD, Kim BE, Gao P, Grant AV, Boguniewicz M, DeBenedetto A, et al. Cytokine modulation of atopic dermatitis filaggrin skin expression. J Allergy Clin Immunol. 2007;120:150-5. (WB)
17. Lucas CR, Cordero-Nieves HM, Erbe RS, McAlees JW, Bhatia S, Hodes RJ, Campbell KS, et al. Prohibitins and the cytoplasmic domain of CD86 cooperate to mediate CD86 signaling in B lymphocytes. J Immunol. 2013;190:723-36. (WB)
18. Hsu H, Baldwin CL, Telfer JC. The endocytosis and signaling of the γδ T cell coreceptor WC1 are regulated by a dileucine motif. J Immunol. 2015;194:2399-406. (In vitro control)
19. Gong J, Yang DJ, Kohanim S, Angelo LS, Kurzrock R. Molecular imaging of gefitinib activity in an epidermal growth factor receptor (EGFR)-bearing xenograft model. Cancer Biol Ther. 2009;8:2237-45. (In vivo control)
20. Xie F, Sun D, Schering A, Nakao S, Zandi S, Liu P, et al. Novel molecular imaging approach for subclinical detection of iritis and evaluation of therapeutic success. Am J Pathol. 2010;177:39-48. (In vivo control)
21. Sun D, Nakao S, Xie F, Zandi S, Bagheri A, Rezaei Kanavi M, et al. Molecular imaging reveals elevated VEGFR-2 expression in retinal capillaries in diabetes: a novel biomarker for early diagnosis. FASEB J. 2014;28:3942-51. (In vivo control)
22. Xia Y, Janda A, Eryilmaz E, Casadevall A, Putterman C. The constant region affects antigen binding of antibodies to DNA by altering secondary structure. Mol Immunol. 2013;56:28-37. (Circular dichroism spectroscopy protein control)
23. French KJ, Coatney RW, Renninger JP, Hu CX, Gales TL, Zhao S, et al. Differences in effects on myocardium and mitochondria by angiogenic inhibitors suggest separate mechanisms of cardiotoxicity. Toxicol Pathol. 2010;38:691-702. (IHC-PS)
24. Hönger G, Hopfer H, Arnold M, Spriewald BM, Schaub S, Amico P. Pretransplant IgG subclasses of donor-specific human leukocyte antigen antibodies and development of antibody-mediated rejection. Transplantation. 2011;92:41-7. (Multiplex)