Hamster Anti-Mouse CD81-UNLB抗體 | 貨號1825-01   規格1.0 mg  (1.0 mg/mL)

產品介紹: CD81，也稱為TAPA-1，是在多種細胞類型上表達的完整膜蛋白(integral membrane protein)，並且在人和小鼠之間具有高度的序列同源性(sequence homology)。 CD81在胸腺基質細胞(thymic stromal cells)上表達，其在γδ+ T細胞向具有αβT細胞受體的更成熟T細胞的轉變中起重要作用。 免疫組織化學染色(Immunohistochemical staining)顯示其表達定位於胸腺的包膜下區域，特別是具有不同聚類模式的細胞。 據推測，CD81的配體是前T細胞受體(pre-T cell receptor)，其由TCRβ鍊(TCR β chain)和糖蛋白pTα(glycoprotein pTα)組成。 單克隆抗體2F7可以在體外阻斷胸腺細胞(thymocyte)與CD81的相互作用。Hamster Anti-Mouse CD81-UNLB 抗體，不具標誌。針對Mouse CD81具特異性。免疫源為Mouse epithelial cell line PAM212。Isotype Control Hamster IgG-UNLB。緩衝液配方在Borate buffered saline, pH 8.2。

 

Chinese hamster ovary cell line CHO-K1 and BALB/cAnN mouse B lymphocyte cell line A20 were stained with Hamster Anti-Mouse CD81-APC (SB Cat. No. 1825-11).

Clone	2F7
Isotype	Hamster (Armenian) IgG3
Isotype Control
Immunogen	Mouse epithelial cell line PAM212
Specificity	Mouse CD81
Alternalte Name(s)	TAPA-1
Description	CD81, also known as TAPA-1, is an integral membrane protein expressed on a variety of cell types and has a high degree of sequence homology between human and mouse. CD81 is expressed on thymic stromal cells where it plays an important role in the transition of γδ+ T cells to more mature T cells with αβ T cell receptors. Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor which is composed of a TCR β chain and glycoprotein pTα. The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro.
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – Flow Cytometry – Quality tested 1,3 Immunohistochemistry –Reported in literature 1 Immunocytochemistry – Reported in literature 5 Immunoprecipitation – Reported in literature 1 Blocking – Reported in literature 1-4 Costimulation – Reported in literature 2
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2795398

引用文獻

References

1. Boismenu R, Rhein M, Fischer WH, Havran WL. A role for CD81 in early T cell development. Science. 1996;271:198-200. (Immunogen, FC, IP, IHC, Block)
2. Takeda Y, He P, Tachibana I, Zhou B, Miyado K, Kaneko H, et al. Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice. J Biol Chem. 2008;283:26089-97. (Block, Costim)
3. Tachibana I, Hemler ME. Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. J Cell Biol. 1999;146:893-904. (FC, Block)
4. Chang Y, Finnemann SC. Tetraspanin CD81 is required for the αvβ5-integrin-dependent particle-binding step of RPE phagocytosis. J Cell Sci. 2007;120:3053-63. (Block)
5.  Kelić S, Levy S, Suarez C, Weinstein DE. CD81 regulates neuron-induced astrocyte cell-cycle exit. Mol Cell Neurosci. 2001;17:551-60. (ICC)