Recombinant Human PDGFA重組蛋白 |貨號Z100367 / Z100368

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Recombinant Human PDGFA重組蛋白 |貨號Z100367
PDGF亞基A,PDGF-1,血小板衍生生長因子A鏈,血小板衍生生長因子α多肽,PDGFA,PDGF-AA,PDGF1
重組人血小板衍生生長因子亞基A(PDGFA)
PDGFA重組蛋白介紹: PDGF家族 (PDGF family) 由四種不同多肽鏈的五種不同的二硫鍵連接的二聚體組成:A,B,C和D (PDGF-AA,PDGF-BB,PDGF-AB,PDGF-CC和PDGF-DD)。主要由巨核細胞合成 (megakaryocytes) ,PDGF存儲在血小板(platelets)的α顆粒中(alpha granules),血小板活化後它們從血小板中釋放。 PDGF受體(PDGF receptors)在成纖維細胞(fibroblasts),成骨細胞(osteoblasts),成軟骨細胞(chondroblasts),平滑肌細胞(smooth muscle cells),神經膠質細胞(glial cells)和內皮細胞(endothelial cells)中表達。 PDGF作為自分泌(autocrine)和旁分泌生長因子(paracrine growth factor)起作用,參與許多生物過程,包括但不限於增生(hyperplasia),趨化性(chemotaxis),胚胎神經元發育(embryonic neuron development),傷口癒合(wound healing )和呼吸小管上皮細胞發育。在血管增生性疾病(vascular proliferative diseases)如動脈粥樣硬化(atherosclerosis)中觀察到PDGF的異常表達。 PDGF調節其自身受體的合成,並且還影響IL1,EGF,5-羥色胺(5-Hydroxytryptamine),LDL和轉鐵蛋白(transferrin)的膜受體的表達。重組人PDGFA是兩條A鏈的二硫鍵連接的同型二聚體。
Gene Symbol | PDGFA |
Gene ID | 5154 |
Accession No. | P04085 |
Source | E. coli |
Appearance | Lyophilized Powder |
Molecular Weight | 14.5 kDa |
Endotoxin Level | <1.0 EU/μg of recombinant protein as determined by the LAL method |
Purity | >95% as determined by SDS-PAGE |
Bioactivity | The ED50 as determined by the dose-dependent proliferation of mouse embryonic fibroblast cells was found to be ≤ 5.0 ng/mL |
Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° – 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |