Goat Anti-Rabbit IgG-HRP抗體 | 貨號4030-05   規格1.0 ML

產品介紹:  Goat Anti-Rabbit IgG-HRP抗體，具HRP標誌。針對Rabbit IgG的heavy chain重鍊具特異性。來源為山羊抗血清。Cross Adsorption Rabbit IgM. Isotype為Goat IgG。緩衝液配方在50% Glycerol/50% Phosphate buffered saline, pH 7.4。

Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.

Specificity	Reacts with the heavy chain of rabbit IgG
Source	Pooled antisera from goats hyperimmunized with rabbit IgG
Cross Adsorption	Rabbit IgM; may react with immunoglobulins from other species
Purification	Affinity chromatography on pooled rabbit IgG covalently linked to agarose
Isotype	Goat IgG
Format/Conjugate	HRP (Horseradish Peroxidase)
Buffer Formulation	50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include – ELISA 1-3 FLISA Other referenced applications for relevant formats include – Flow Cytometry 4,5 Immunohistochemistry-Frozen Sections 6-8 Immunohistochemistry-Paraffin Sections 7,9-11 Immunocytochemistry 12-14 Western Blot 15-25
Recommended Dilutions	Please refer to product specific

References

1. Wimer-Mackin S, Hinchcliffe M, Petrie CR, Warwood SJ, Tino WT, Williams MS, et al. An intranasal vaccine targeting both the Bacillus anthracis toxin and bacterium provides protection against aerosol spore challenge in rabbits. Vaccine. 2006;24:3953-63. (ELISA)
2. Beauchemin C, Moerke NJ, Faloon P, Kaye KM. Assay development and high-throughput screening for inhibitors of Kaposi’s sarcoma-associated herpesvirus N-terminal latency-associated nuclear antigen binding to nucleosomes. J Biomol Screen. 2014;19:947-58. (ELISA)
3. Li H, Fu Y, Wu Q, Zhou Y, Crossman DK, Yang P, et al. Interferon-induced mechanosensing defects impede apoptotic cell clearance in lupus. J Clin Invest. 2015;125:2877-90. (ELISA)
4. Sehgal D, Mage RG, Schiaffella E. VH mutant rabbits lacking the VH1a2 gene develop a2+ B cells in the appendix by gene conversion-like alteration of a rearranged VH4 gene. J Immunol. 1998;160:1246-55. (FC)
5. Yang J, Pospisil R, Mage RG. Expression and localization of rabbit B-cell activating factor (BAFF) and its specific receptor BR3 in cells and tissues of the rabbit immune system. Dev Comp Immunol. 2009;33:697-708. (FC)
6. Schiaffella E, Sehgal D, Anderson AO, Mage RG. Gene conversion and hypermutation during diversification of VH sequences in developing splenic germinal centers of immunized rabbits. J Immunol. 1999;162:3984-95 (IHC-FS)
7. Dasso JF, Obiakor H, Bach H, Anderson AO, Mage RG. A morphological and immunohistological study of the human and rabbit appendix for comparison with the avian bursa. Dev Comp Immunol. 2000;24:797-814. (IHC-FS, IHC-PS)
8. Mau M, Kaiser TM, Südekum K. Carbonic anhydrase II is secreted from bovine parotid glands. Histol Histopathol. 2010;25:321-9. (IHC-FS)
9. Gaiser T, Berroa-Garcia L, Kemmerling R, Dutta A, Ried T, Heselmeyer-Haddad K. Automated analysis of protein expression and gene amplification within the same cells of paraffin-embedded tumour tissue. Anal Cell Pathol. 2010;33:105-12. (IHC-PS)
10. Gaiser T, Camps J, Meinhardt S, Wangsa D, Nguyen QT, Varma S, et al. Genome and transcriptome profiles of CD133-positive colorectal cancer cells. Am J Pathol. 2011;178:1478-88. (IHC-PS)
11. Pasternak JA, Kent-Dennis C, Van Kessel AG, Wilson HL. Claudin-4 undergoes age-dependent change in cellular localization on pig jejunal villous epithelial cells, independent of bacterial colonization. Mediators Inflamm. 2015;2015:263629. (IHC-PS)
12. Hansen SH, Sandvig K, van Deurs B. Clathrin and HA2 adaptors: effects of potassium depletion, hypertonic medium, and cytosol acidification. J Cell Biol. 1993;121:61-72. (ICC)
13. Duan Y, Huang S, Yang J, Niu P, Gong Z, Liu X, et al. HspA1A facilitates DNA repair in human bronchial epithelial cells exposed to Benzo[a]pyrene and interacts with casein kinase 2. Cell Stress Chaperones. 2014;19:271-9. (ICC)
14. Hull JJ, Wang M. Molecular cloning and characterization of G alpha proteins from the western tarnished plant bug, Lygus hesperus. Insects. 2015;6:54-76. (ICC)
15. Park S, Gaur AB, Lengyel E, Peter ME. The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2. Genes Dev. 2008;22:894-907. (WB)
16. Røsjø H, Husberg C, Dahl MB, Stridsberg M, Sjaastad I, Finsen AV, et al. Chromogranin B in heart failure: a putative cardiac biomarker expressed in the failing myocardium. Circ Heart Fail. 2010;3:503-11. (WB)
17. Lunde IG, Kvaløy H, Austbø B, Christensen G, Carlson CR. Angiotensin II and norepinephrine activate specific calcineurin-dependent NFAT transcription factor isoforms in cardiomyocytes. J Appl Physiol. 2011;111:1278-89. (WB)
18. van Gent M, Braem SG, de Jong A, Delagic N, Peeters JG, Boer IG, et al. Epstein-Barr virus large tegument protein BPLF1 contributes to innate immune evasion through interference with toll-like receptor signaling. PLoS Pathog. 2014;10(2):e1003960. (WB)
19. Gao H, Liebenthal DA, Yallampalli U, Yallampalli C. Adrenomedullin promotes rat trophoblast stem cell differentiation. Biol Reprod. 2014;91:65. (WB)
20. Vistnes M, Aronsen JM, Lunde IG, Sjaastad I, Carlson CR, Christensen G. Pentosan polysulfate decreases myocardial expression of the extracellular matrix enzyme ADAMTS4 and improves cardiac function in vivo in rats subjected to pressure overload by aortic banding. PLoS One. 2014;9(3):e89621. (WB)
21. Muller M, Hutin S, Marigold O, Li KH, Burlingame A, Glaunsinger BA. A ribonucleoprotein complex protects the interleukin-6 mRNA from degradation by distinct herpesviral endonucleases. PLoS Pathog. 2015;11(5):e1004899. (WB)
22. Pedro JM, Wei Y, Sica V, Maiuri MC, Zou Z, Kroemer G, et al. BAX and BAK1 are dispensable for ABT-737-induced dissociation of the BCL2-BECN1 complex and autophagy. Autophagy. 2015;11:452-9. (WB)
23. Graham MK, Brown TR, Miller PS. Targeting the human androgen receptor gene with platinated triplex-forming oligonucleotides. Biochemistry. 2015;54:2270-82. (WB)
24. Aziz M, Yang W, Corbo LM, Chaung WW, Matsuo S, Wang P. MFG-E8 inhibits neutrophil migration through αvβ3-integrin-dependent MAP kinase activation. Int J Mol Med. 2015;36:18-28. (WB)
25. Tang B, Zhou W, Du J, He Y, Li Y. Identification of human leukemia antigen A*0201-restricted epitopes derived from epidermal growth factor pathway substrate number 8. Mol Med Rep. 2015;12:1741-52. (WB)

 

細胞免疫專家極力推薦SouthernBiotech高品質二級抗體

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全，包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質（FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）各種螢光標誌、另具酵素（HRP、AP）、或未標誌及Low Endotoxin/Azide Free（LE/AF）等標定。

Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |

Cat. No.	Product Description	Clone	Size
4010-05	Goat Anti-Rabbit Ig, Human ads-HRP		1.0 mL
4020-05	Goat Anti-Rabbit IgM-HRP		1.0 mL
4030-05	Goat Anti-Rabbit IgG-HRP		1.0 mL
4040-05	Goat Anti-Rabbit IgG Fab-HRP		1.0 mL
4041-05	Goat Anti-Rabbit IgG Fc-HRP		1.0 mL
4049-05	Goat Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-HRP		1.0 mL
4050-05	Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP		1.0 mL
4051-05	Goat Anti-Rabbit IgG(H+L), Human ads-HRP		1.0 mL
4052-05	Goat F(ab’)2 Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP		1.0 mL
4054-05	Goat F(ab’)2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-HRP		1.0 mL
4055-05	Goat Anti-Rabbit IgG(H+L)-HRP		1.0 mL
4058-05	Goat Anti-Rabbit IgG Fab, Mouse/Human SP ads-HRP		1.0 mL
4060-05	Mouse Anti-Rabbit Light Chain-HRP	SB62a	1.0 mL
4065-05	Rat Anti-Rabbit Ig-HRP	SB87a	1.0 mL
4090-05	Mouse Anti-Rabbit IgG-HRP	2A9	1.0 mL
6310-05	Swine Anti-Rabbit IgG(H+L), Mouse/Rat/Human/Goat SP ads-HRP		1.0 mL
6440-05	Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-HRP		1.0 mL
6441-05	Donkey Anti-Rabbit IgG(H+L)-HRP		1.0 mL
6442-05	Donkey Anti-Rabbit IgG(H+L), Multi-Species SP ads-HRP		1.0 mL
6444-05	Donkey F(ab’)2 Anti-Rabbit IgG(H+L), Multi-Species SP ads-HRP		1.0 mL
6446-05	Donkey F(ab’)2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-HRP		1.0 mL