CaspGLOW™螢光素活性Caspase-9染色試劑盒 | 貨號K189

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CaspGLOW™螢光素活性Caspase-9染色試劑盒 | 貨號K189
CaspGLOW™ Fluorescein Active Caspase-9 Staining Ki-Detects activated Caspase-9 by FACS, FL or plate reader
產品描述:Caspases半胱天冬酶的激活在apoptosis細胞凋亡中起重要作用。 CaspGLOW™螢光素活性Caspase-9染色試劑盒,為活細胞中活化的caspase-9的靈敏檢測提供了便利的方法。 該測定使用與FITC綴合的半胱天冬酶-9抑制劑LEHD-FMK(FITC-LEHD-FMK)作為螢光標記物。 FITC-LEHD-FMK是細胞可滲透的、無毒的、並且在凋亡細胞中不可逆地結合活化的胱天蛋白酶-9。
| Detection Method | Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm) |
| Species Reactivity | Mammalian |
| Applications | Sensitive detection of activated caspase-9 in living cells. |
| Features & Benefits | • Simple one-step procedure; takes only 1-2 hours • Fast and convenient • The FITC label allows for direct detection of the activated caspases in apoptotic cells |
| Kit Components | • FITC-LEHD-FMK • Wash Buffer • Z-VAD-FMK |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
引用文獻
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Désio Aurélio Farias-de-Oliveira et al., Caspase-8 and caspase-9 mediate thymocyte apoptosis in Trypanosoma cruzi acutely infected mice. J. Leukoc. Biol., Feb 2013; 93: 227 – 234
Zhnag, X. et. al. Interferon-Description: {gamma} Exacerbates Dry Eye–Induced Apoptosis in Conjunctiva through Dual Apoptotic Pathways. Invest. Ophthalmol. Vis. Sci., Aug 2011; 52: 6279 – 6285.
Jamadar-Shroff V et al (2009) Clin. Cancer Res.; 15: 1269 – 1276.
Ito, E. et al. Potential Use of Cetrimonium Bromide as an Apoptosis-Promoting Anticancer Agent for Head and Neck Cancer. Mol. Pharmacol. 2009; 76: 969-983.
