還原型穀胱甘肽(GSH)檢測 | Reduced Glutathione (GSH) Assay Kit貨號K740-100

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PicoProbe™還原型穀胱甘肽(GSH)檢測試劑盒(螢光) | 貨號K740-100
PicoProbe™ Reduced Glutathione (GSH) Assay Kit (Fluorometric) It specifically detects reduced glutathione. Oxidized GSSG is not detected.
產品描述:Glutathione穀胱甘肽(GSH)是一種 thiol-containing tripeptide含巰基的三肽,是生命系統中的關鍵抗氧化劑。 細胞內GSH狀態似乎是細胞總體健康狀況的指標。 BioVision的PicoProbe™還原型穀胱甘肽檢測試劑盒基於GSH和螢光團存在的specific enzymatic cycling method 特定酶循環方法。 螢光團的還原產生穩定的螢光產物,其螢光與樣品中GSH的量成正比,followed kinetically動力學(Ex / Em = 535 / 587nm)。 我們的檢測方法是市場上最具特異性和最敏感的。 Oxidized Glutathione氧化型穀胱甘肽不會干擾測定。 該測定簡單,可重複,並且可以在100μl反應中特異性地檢測低至8pmol /孔的還原形式的穀胱甘肽(GSH)。



| Cat # +Size | K740-100 |
| Size | 100 assays |
| Kit Summary | • Detection method- Fluorescence (535/587 nm) • Species reactivity- Mammalian • Applications- The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected. |
| Detection Method | Detection method- Fluorescence (Ex/Em 535/587 nm) |
| Species Reactivity | Mammalian |
| Applications | The Fluorometric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected. |
| Features & Benefits | • Simple procedure; takes only ~1-2 hours • Fast and convenient • Specific: does not detect oxidized GSSG • Sensitive: detects as low as 8 pmol GSH |
| Kit Components | • GSH Assay Buffer • PicoProbeTM (in DMSO) • Substrate Mix A • Substrate Mix B • Enzyme Mix A • Enzyme Mix B • Enzyme Mix C • Sulfosalicylic Acid (SSA, 1 gram) • GSH Standard |
