細胞轉化分析試劑盒(比色法)| Cell Transformation Assay Kit貨號K921-100

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特點
Cell Transformation Assay Kit (Colorimetric) more sensitive than the Soft agar assay/ Colony formation assay
產品描述:Transformed cells轉化的細胞可以proliferate增殖而不附著在表面上。 錨定獨立細胞生長是細胞轉化的標誌。 Soft-Agar Assay/ Colony formation assay是一種用於體外篩選transformation細胞轉化的傳統方法。 然而,這種方法很長(3-4 weeks incubatio),laborious費力(counting colonies計數菌落)和inconsistent不一致(由於主觀計數)。BioVision的Cell Transformation Assay細胞轉化分析比傳統的soft-agar assay軟瓊脂分析更快,更穩定,更靈敏。 該試劑盒基於通過細胞線粒體脫氫酶將四唑鹽(WST-8)轉化為formazan. 產生的信號與活細胞的數量成正比。 這種一步性法非放射性法操作簡單(只需添加和讀取,不需要收穫細胞和溶解步驟)。 該測定具有高通量適應性,並具有10000-400000個細胞的線性範圍。 整個細胞轉化分析可在7-8天內完成。

| Cat # +Size | K921-100 |
| Size | 100 assays |
| Detection Method | Colorimetric (OD 450 nm) |
| Species Reactivity | Mammalian cells |
| Applications | • Measure cell transformation in response to stimuli • Screen and characterize compounds that influence cell transformation |
| Features & Benefits | • Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation |
| Kit Components | • Agarose Powder DMEM Solution (10X) • Staining Solution • WST Reagent • Electro Coupling Solution (ECS) |
| Storage Conditions | -20°C |
