精氨酸酶活性比色分析試劑盒 | 貨號K755-100 (Abcam ab180877) 0.2U / L

Arginase Activity Colorimetric Assay Kit

產品描述：Arginase精氨酸酶（EC 3.5.3.1）是Urea Cycle尿素循環的最終酶。 它將L-精氨酸轉化為尿素和L-鳥氨酸，在去除體內銨離子方面起著重要作用。 精氨酸酶有兩種同種型：精氨酸酶I和精氨酸酶II。Arginase I  精氨酸酶I主要存在於肝臟中並且在urea cycle 尿素循環中起重要作用，而Arginase II精氨酸酶II存在於 kidney 腎臟和其他組織中並調節Arginine精氨酸/Ornithine鳥氨酸濃度。 Arginase deficiency精氨酸酶缺乏可導致嚴重的症狀，包括 neurological impairment神經損傷，dementia癡呆和hyperammonemia高氨血症。 精氨酸酶活性的分析是尿素代謝途徑研究的基礎。 BioVision的精氨酸酶活性檢測試劑盒簡單，靈敏，快速。 在該測定中，精氨酸酶與精氨酸反應並經歷一系列反應以形成中間體，該中間體與OxiRed ^TM探針按化學計量反應以產生有色產物（OD 570nm）。 該試劑盒可以在96孔測定形式中檢測小於0.2U / L的精氨酸酶活性。

Arginase (EC 3.5.3.1) is the final enzyme of the Urea Cycle. It converts L-arginine into urea and L-ornithine and plays an important role in removing ammonium ion from the body. Arginase has two isoforms: Arginase I and Arginase II. Arginase I is mainly present in the liver and plays an important part in the urea cycle whereas Arginase II is present in kidney and other tissues and regulates Arginine/Ornithine concentration. Arginase deficiency can lead to severe symptoms including neurological impairment, dementia and hyperammonemia. Analysis of Arginase activity is fundamental to the study of the urea metabolic pathway. BioVision’s Arginase Activity Assay kit is simple, sensitive and rapid. In this assay, Arginase reacts with arginine & undergoes a series of reactions to form an intermediate that reacts stoichiometrically with OxiRed™ Probe to generate the colored product (OD 570 nm). The kit can detect Arginase activity less than 0.2 U/L in 96-well assay format.

Cat # +Size	K755-100
Size	100 assays
Detection Method	Absorbance (570 nm)
Applications	Measurement of Arginase Activity in various tissues/cells Analysis of urea cycle disorders
Features & Benefits	• Simple, rapid & convenient • The assay can detect Arginase Activity less than 0.2U/L in 96-well assay format.
Kit Components	• Arginase Assay Buffer • OxiRed™ Probe (in DMSO) • Arginase Substrate (Lyophilized) • Arginase Enzyme Mix (Lyophilized) • Arginase Developer (Lyophilized) • Arginase Converter Enzyme (Lyophilized) • H2O2 Standard (0.88 M) • Arginase Positive Control (Lyophilized)