Goat Anti-Human Ig-Alexa Fluor® 647  | 貨號2010-31   規格1.0 mL  (1.0 mg / mL)

產品介紹: Goat Anti-Human IgG，來源為Pooled antisera from goats hyperimmunized with human IgG, IgM, and IgA。特異性辨視of human IgG, IgM, and IgA s之重鏈heavy 、 與輕鏈light chains。此抗體Affinity chromatography on immunoglobulins covalently linked to agarose純化, 具AF647 (Alexa Fluor® 647)標誌。可應用於各種免疫分析的試驗。

Specificity	Reacts with the heavy and light chains of human IgG, IgM, and IgA
Source	Pooled antisera from goats hyperimmunized with human IgG, IgM, and IgA
Cross Adsorption	None; may react with immunoglobulins from other species
Purification	Affinity chromatography on pooled human immunoglobulins covalently linked to agarose
Isotype	Goat IgG
Isotype Control	Goat IgG-Alexa Fluor® 647
Format/Conjugate	AF647 (Alexa Fluor® 647)
Buffer Formulation	Supplied in PBS/NaN3
Concentration	1.0 mg/mL
Volume	1.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include – ELISA 1-8 FLISA Flow Cytometry 15,16,18 Other referenced applications for relevant formats include – Immunocytochemistry 9 Western Blot 10-13 Multiplex 14 Stimulation 15-17
Recommended Dilutions	Please refer to product specific Technical Bulletin

References

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2. Pancook JD, Beuerlein G, Pecht G, Tang Y, Nie Y, Wu H, et al. In vitro affinity maturation of human IgM antibodies reactive with tumor-associated antigens. Hybrid Hybridomics. 2001;20:383-96. (ELISA)
3. Weitkamp J, Kallewaard N, Kusuhara K, Feigelstock D, Feng N, Greenberg HB, et al. Generation of recombinant human monoclonal antibodies to rotavirus from single antigen-specific B cells selected with fluorescent virus-like particles. J Immunol Methods. 2003;275:223-37. (ELISA)
4. Ohdan H, Zhou W, Tanaka Y, Irei T, Fuchimoto Y, Egawa H, et al. Evidence of immune tolerance to blood group antigens in a case of ABO-incompatible pediatric liver transplantation. Am J Transplant. 2007;7:2190-4. (ELISA)
5. Williams JV, Weitkamp J, Blum DL, LaFleur BJ, Crowe JE Jr. The human neonatal B cell response to respiratory syncytial virus uses a biased antibody variable gene repertoire that lacks somatic mutations. Mol Immunol. 2009;47:407-14. (ELISA)
6. Fan Q, Longnecker R. The Ig-like V-type domain of paired Ig-like type 2 receptor alpha is critical for herpes simplex virus type 1-mediated membrane fusion. J Virol. 2010;84:8664-72. (ELISA)
7. Yang G, Holl TM, Liu Y, Li Y, Lu X, Nicely NI, et al. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med. 2013;210:241-56. (ELISA)
8. Martí GP, Mohn KG, Cox RJ, Brokstad KA. The influence of tonsillectomy on total serum antibody levels. Scand J Immunol. 2014;80:377-9. (ELISA)
9. Moody MA, Yates NL, Amos JD, Drinker MS, Eudailey JA, Gurley TC, et al. HIV-1 gp120 vaccine induces affinity maturation in both new and persistent antibody clonal lineages. J Virol. 2012;86:7496-507. (ICC)
10. Moritz DR, Gheyselinck J, Klemenz R. Expression analysis of the soluble and membrane-associated forms of the interleukin-1 receptor-related Tl protein in primary mast cells and fibroblasts. Hybridoma. 1998;17:107-16. (WB)
11. Davenport V, Guthrie T, Findlow J, Borrow R, Williams NA, Heyderman RS. Evidence for naturally acquired T cell-mediated mucosal immunity to Neisseria meningitidis. J Immunol. 2003;171:4263-70. (WB)
12. Botelho-Nevers E, Gouriet F, Lepidi H, Couvret A, Amphoux B, Dessi P, et al. Chronic nasal infection caused by Klebsiella rhinoscleromatis or Klebsiella ozaenae: two forgotten infectious diseases. Int J Infect Dis. 2007;11:423-9. (WB)
13. Parcina M, Miranda-Garcia MA, Durlanik S, Ziegler S, Over B, Georg P, et al. Pathogen-triggered activation of plasmacytoid dendritic cells induces IL-10-producing B cells in response to Staphylococcus aureus. J Immunol. 2013;190:1591-602. (WB)
14. Pochechueva T, Chinarev A, Bovin N, Fedier A, Jacob F, Heinzelmann-Schwarz V. PEGylation of microbead surfaces reduces unspecific antibody binding in glycan-based suspension array. J Immunol Methods. 2014;412:42-52. (Multiplex)
15. Fruehling S, Lee SK, Herrold R, Frech B, Laux G, Kremmer E, et al. Identification of latent membrane protein 2A (LMP2A) domains essential for the LMP2A dominant-negative effect on B-lymphocyte surface immunoglobulin signal transduction. J Virol. 1996;70:6216-26. (FC, Stim)
16. Fruehling S, Swart R, Dolwick KM, Kremmer E, Longnecker R. Tyrosine 112 of latent membrane protein 2A is essential for protein tyrosine kinase loading and regulation of Epstein-Barr virus latency. J Virol. 1998;72:7796-806. (FC, Stim)
17. Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, et al. Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011;90:5-19. (Stim)
18. Wiehr S, Bühler P, Gierschner D, Wolf P, Rolle A, Kesenheimer C, et al. Pharmacokinetics and PET imaging properties of two recombinant anti-PSMA antibody fragments in comparison to their parental antibody. Prostate. 2014;74:743-55. (FC)