鹼性磷酸酶螢光活性檢測 | Alkaline Phosphatase, ALP 貨號K422-500 (ab83371)

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鹼性磷酸酶(ALP)螢光活性分析套組 | 貨號K422-500
Alkaline Phosphatase Activity Colorimetric Assay Kit (ab83371)
產品描述:鹼性磷酸酶(ALP)催化鹼性緩衝液中磷酸酯的水解,產生有機自由基和無機磷酸鹽。 鹼性磷酸酶水平和活性的變化與肝臟和骨骼中的許多疾病相關。 鹼性磷酸酶也是ELISA中與二抗結合的流行酶。 在BioVision的鹼性磷酸酶螢光測定試劑盒中,ALP切割非螢光4-甲基繖形酮磷酸二鈉鹽(MUP)底物的磷酸基團,產生強螢光信號(Ex / Em = 360nm / 440nm)。 該試劑盒是一種超靈敏,簡單,直接和HTS檢測方法,用於測量血清和生物樣品中的ALP活性,檢測靈敏度約為1μU,比比色檢測更靈敏。 該試劑盒適用於研究和drug discovery。

| Cat # +Size | K422-500 |
| Size | 500 assays |
| Detection Method | Fluorescence (360/440) |
| Species Reactivity | Mammalian |
| Applications | The assay designed to measure ALP activity in serum and bio-samples with detection sensitivity ~1 µU |
| Features & Benefits | • Simple procedure; takes less than 1 hour • Fast and convenient • The kit is an ultra-sensitive, direct and HTS-ready fluorometric assay designed to measure AP activity in serum and other bio-samples. |
| Kit Components | • ALP Assay Buffer • MUP Substrate • ALP Enzyme • Stop Solution |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
