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Goat Anti-Mouse IgG(H+L), Human ads-HRP | SouthernBiotech貨號1031-05

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Goat Anti-Mouse IgG(H+L)抗體操作手冊

特點

Goat Anti-Mouse IgG(H+L), Human ads-HRP | 貨號1031-05

細胞免疫專家極力推薦SouthernBiotech高品質二級抗體

美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全,包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質(FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)各種螢光標誌、另具酵素(HRP、AP)、或未標誌及Low Endotoxin/Azide Free(LE/AF)等標定。

ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Mouse F(ab’)2 Anti-Human IgM-UNLB (SB Cat. No. 9023-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05).

CHO-K1 cell lysates transfected with GFP – 6X His-tag were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-UNLB (SB Cat. No. 4603-01L) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05) secondary antibody and chemiluminescent detection.

Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05), DAB, and hematoxylin.

 

Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3and with the light chains of mouse IgM and IgA
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG covalently linked to agarose
Isotype Goat IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include –
ELISA 1-7
FLISA 8
Flow Cytometry 3,8-13
Other referenced applications for relevant formats include –
ELISpot 28
Immunohistochemistry-Frozen Sections 14,15
Immunohistochemistry-Paraffin Sections 16
Immunocytochemistry 12,17-20
Western Blot 21-27
Recommended Dilutions Please refer to product specific Technical Bulletin

References

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  2. Stegmann T, Kamphuis T, Meijerhof T, Goud E, de Haan A, Wilschut J. Lipopeptide-adjuvanted respiratory syncytial virus virosomes: A safe and immunogenic non-replicating vaccine formulation. Vaccine. 2010;28:5543-50. (ELISA)
  3. Christianson GJ, Sun VZ, Akilesh S, Pesavento E, Proetzel G, Roopenian DC. Monoclonal antibodies directed against human FcRn and their applications. MAbs. 2012;4:208-16. (ELISA, FC)
  4. Stefka AT, Feehley T, Tripathi P, Qiu J, McCoy K, Mazmanian SK, et al. Commensal bacteria protect against food allergen sensitization. Proc Natl Acad Sci USA. 2014;111:13145-50. (ELISA)
  5. Silva-Sanchez A, Liu CR, Vale AM, Khass M, Kapoor P, Elgavish A, et al. Violation of an evolutionarily conserved immunoglobulin diversity gene sequence preference promotes production of dsDNA-specific IgG antibodies. PLoS One. 2015;10(2):e0118171. (ELISA)
  6. Lascano V, Hahne M, Papon L, Cameron K, Röeder C, Schafmayer C, et al. Circulating APRIL levels are correlated with advanced disease and prognosis in rectal cancer patients. Oncogenesis. 2015;4:e136. (ELISA)
  7. Zhao H, Bauzon F, Bi E, Yu JJ, Fu H, Lu Z, et al. Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age. J Biol Chem. 2015;290:5797-809. (ELISA)
  8. Kilpatrick KE, Cutler T, Whitehorn E, Drape RJ, Macklin MD, Witherspoon SM, et al. Gene gun delivered DNA-based immunizations mediate rapid production of murine monoclonal antibodies to the Flt-3 receptor. Hybridoma. 1998;17:569-76. (FLISA, FC)
  9. Johnson SE, Shah N, Panoskaltsis-Mortari A, LeBien TW. Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells. J Immunol. 2005;175:7325-31. (FC)
  10. Donini M, Zenaro E, Tamassia N, Dusi S. NADPH oxidase of human dendritic cells: role in Candida albicans killing and regulation by interferons, dectin-1 and CD206. Eur J Immunol. 2007;37:1194-203. (FC)
  11. Delaleu N, Madureira AC, Immervoll H, Jonsson R. Inhibition of experimental Sjögren’s syndrome through immunization with HSP60 and its peptide amino acids 437-460. Arthritis Rheum. 2008;58:2318-28. (FC)
  12. Le TL, Mironova E, Garcin D, Compans RW. Induction of influenza-specific mucosal immunity by an attenuated recombinant Sendai virus. PLoS One. 2011;6:e18780. (FC, ICC)
  13. Daflon-Yunes N, Pinto-Silva FE, Vidal RS, Novis BF, Berguetti T, Lopes RR, et al. Characterization of a multidrug-resistant chronic myeloid leukemia cell line presenting multiple resistance mechanisms. Mol Cell Biochem. 2013;383:123-35. (FC)
  14. Vainio O, Dunon D, Aïssi F, Dangy J, McNagny KM, Imhof BA. HEMCAM, an adhesion molecule expressed by c-kit+ hemopoietic progenitors. J Cell Biol. 1996;135:1655-68. (IHC-FS)
  15. Xia Y, Pawar RD, Nakouzi AS, Herlitz L, Broder A, Liu K, et al. The constant region contributes to the antigenic specificity and renal pathogenicity of murine anti-DNA antibodies. J Autoimmun. 2012;39:398-411. (IHC-FS)
  16. Deckert V, Kretz B, Habbout A, Raghay K, Labbé J, Abello N, et al. Development of abdominal aortic aneurysm is decreased in mice with plasma phospholipid transfer protein deficiency. Am J Pathol. 2013;183:975-86. (IHC-PS)
  17. Scaife S, Brown R, Kellie S, Filer A, Martin S, Thomas AM, et al. Detection of differentially expressed genes in synovial fibroblasts by restriction fragment differential display. Rheumatology. 2004;43:1346-52. (ICC)
  18. Thomas RA, Norman JC, Huynh TT, Williams B, Bolton SJ, Wardlaw AJ. Mechanical stretch has contrasting effects on mediator release from bronchial epithelial cells, with a rho-kinase-dependent component to the mechanotransduction pathway. Respir Med. 2006;100:1588-97. (ICC)
  19. Lee S, Bahn JH, Choi CK, Whitlock NC, English AE, Safe S, et al. ESE-1/EGR-1 pathway plays a role in tolfenamic acid-induced apoptosis in colorectal cancer cells. Mol Cancer Ther. 2008;7:3739-50. (ICC)
  20. Tarafdar S, Poe JA, Smithgall TE. The accessory factor Nef links HIV-1 to Tec/Btk kinases in an Src homology 3 domain-dependent manner. J Biol Chem. 2014;289:15718-28. (ICC)
  21. Dalen KT, Ulven SM, Arntsen BM, Solaas K, Nebb HI. PPARα activators and fasting induce the expression of adipose differentiation-related protein in liver. J Lipid Res. 2006;47:931-43. (WB)
  22. Hangen E, De Zio D, Bordi M, Zhu C, Dessen P, Caffin F, et al. A brain-specific isoform of mitochondrial apoptosis-inducing factor: AIF2. Cell Death Differ. 2010;17:1155-66. (WB)
  23. Lunde IG, Kvaløy H, Austbø B, Christensen G, Carlson CR. Angiotensin II and norepinephrine activate specific calcineurin-dependent NFAT transcription factor isoforms in cardiomyocytes. J Appl Physiol. 2011;111:1278-89. (WB)
  24. Norheim F, Raastad T, Thiede B, Rustan AC, Drevon CA, Haugen F. Proteomic identification of secreted proteins from human skeletal muscle cells and expression in response to strength training. Am J Physiol Endocrinol Metab. 2011;301:E1013-21. (WB)
  25. Thomas AP, Dunn TN, Drayton JB, Oort PJ, Adams SH. A high calcium diet containing nonfat dry milk reduces weight gain and associated adipose tissue inflammation in diet-induced obese mice when compared to high calcium alone. Nutr Metab. 2012;9:3. (WB)
  26. Stuckenholz C, Lu L, Thakur PC, Choi T, Shin D, Bahary N. Sfrp5 modulates both Wnt and BMP signaling and regulates gastrointestinal organogenesis in the zebrafish, Danio rerio. PLoS One. 2013;8(4):e62470. (WB)
  27. Muller M, Hutin S, Marigold O, Li KH, Burlingame A, Glaunsinger BA. A ribonucleoprotein complex protects the interleukin-6 mRNA from degradation by distinct herpesviral endonucleases. PLoS Pathog. 2015;11(5):e1004899. (WB)

28.   Rahman ZS, Niu H, Perry D, Wakeland E, Manser T, Morel L. Expression of the autoimmune Fcgr2b NZW allele fails to be upregulated in germinal center B cells and is associated with increased IgG production. Genes Immun. 2007;8:604-12. (ELISPOT)

 

 Cat. No. Product Description Size
1031-01  Goat Anti-Mouse IgG(H+L), Human ads-UNLB 1.0 mg
1031-02  Goat Anti-Mouse IgG(H+L), Human ads-FITC 1.0 mg
1031-03  Goat Anti-Mouse IgG(H+L), Human ads-TRITC 1.0 mg
1031-04  Goat Anti-Mouse IgG(H+L), Human ads-AP 1.0 mL
1031-05  Goat Anti-Mouse IgG(H+L), Human ads-HRP 1.0 mL
1031-07  Goat Anti-Mouse IgG(H+L), Human ads-TXRD 1.0 mg
1031-08  Goat Anti-Mouse IgG(H+L), Human ads-BIOT 1.0 mg
1031-09  Goat Anti-Mouse IgG(H+L), Human ads-PE 0.5 mg
1031-09S  Goat Anti-Mouse IgG(H+L), Human ads-PE 0.25 mg
1031-11L  Goat Anti-Mouse IgG(H+L), Human ads-APC 0.5 mg
1031-11S  Goat Anti-Mouse IgG(H+L), Human ads-APC 0.25 mg
1031-15  Goat Anti-Mouse IgG(H+L), Human ads-CY5 1.0 mg
1031-30  Goat Anti-Mouse IgG(H+L), Human ads-Alexa Fluor®488 1.0 mg
1031-31  Goat Anti-Mouse IgG(H+L), Human ads-Alexa Fluor®647 1.0 mg
1031-32  Goat Anti-Mouse IgG(H+L), Human ads-Alexa Fluor®555 1.0 mg

Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |

 

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