抗小鼠MHC II-SPRD  | 貨號1895-13    

Rat Anti-Mouse MHC Class II-SPRD (Spectral Red® is a PE/CY5 tandem conjugate)

BALB/c mouse splenocytes were stained with Rat Anti-Mouse MHC Class II-SPRD (SB Cat. No. 1895-13) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).

產品介紹: 單克隆抗體NIMR-4與MHC II類抗原上的非多態性I-A編碼表位特異性反應。 II類抗原主要在抗原呈遞細胞上表達，包括B淋巴細胞，巨噬細胞，樹突細胞和某些上皮細胞。Southernbiotech提供高品質Rat Anti-Mouse MHC Class II-SPRD抗體，對Mouse MHC Class II具有專一性，Isotype為Rat IgG_2b-SPRD。抗體純化後具有SPRD (Spectral Red® is a PE/CY5 tandem conjugate) 標誌。抗體溶液為Supplied in PBS/NaN3 and a stabilizing agent。貯存於2-8°C。0.1 mg/mL, 1ml包裝。

Clone	NIMR-4
Isotype	Rat IgG2bκ
Isotype Control	Rat IgG2b-SPRD
Specificity	Mouse MHC Class II
Alternalte Name(s)	I-A antigen
Description	The monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells, and certain epithelial cells.
Format/Conjugate	SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation	Supplied in PBS/NaN3 and a stabilizing agent
Concentration	0.1 mg/mL
Volume	1.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – Flow Cytometry – Quality tested 4-13 Immunohistochemistry-Frozen Sections – Reported in literature 1 Immunocytochemistry – Reported in literature 1,2 Separation – Reported in literature 3

References

1. Andrew EM, Parkhouse RM. Immune induction of Ia antigens in activated T cells and in kidney epithelial cells in mice. Immunology. 1986;58:603-6. (IHC-FS, ICC)
2. Zaliauskiene L, Kang S, Sparks K, Zinn KR, Schwiebert LM, Weaver CT, et al. Enhancement of MHC class II-restricted responses by receptor-mediated uptake of peptide antigens. J Immunol. 2002;169:2337-45. (ICC)
3. Lindsey JW. Dexamethasone-induced Ras-related protein 1 is a potential regulatory protein in B lymphocytes. Int Immunol. 2007;19:583-90. (Sep)
4. Caspar-Bauguil S, Cousin B, Galinier A, Segafredo C, Nibbelink M, André M, et al. Adipose tissues as an ancestral immune organ: site-specific change in obesity. FEBS. 2005;579:3487-92. (FC)
5. Bauer C, Bauernfeind F, Sterzik A, Orban M, Schnurr M, Lehr HA, et al. Dendritic cell-based vaccination combined with gemcitabine increases survival in a murine pancreatic carcinoma model. Gut. 2007;56:1275-82. (FC)
6. Krishnamoorthy N, Oriss TB, Paglia M, Fei M, Yarlagadda M, Vanhaesebroeck B, et al. Activation of c-Kit in dendritic cells regulates T helper cell differentiation and allergic asthma. Nat Med. 2008;14:565-73. (FC)
7. Gu T, Zhu Y, Chen C, Li M, Chen Y, Yu G, et al. Fine-tuned expression of programmed death 1 ligands in mature dendritic cells stimulated by CD40 ligand is critical for the induction of an efficient tumor specific immune response. Cell Mol Immunol. 2008;5:33-9. (FC)
8. Brix S, Lund P, Kjaer TM, Straarup EM, Hellgren LI, Frøkiær H. CD4+ T-cell activation is differentially modulated by bacteria-primed dendritic cells, but is generally down-regulated by n-3 polyunsaturated fatty acids. Immunology. 2010;129:338-50. (FC)
9. Jacobsen JT, Lunde E, Sundvold-Gjerstad V, Munthe LA, Bogen B. The cellular mechanism by which complementary Id+ and anti-Id antibodies communicate: T cells integrated into idiotypic regulation. Immunol Cell Biol. 2010;88:515-22. (FC)
10. Arora M, Poe SL, Oriss TB, Krishnamoorthy N, Yarlagadda M, Wenzel SE, et al. TLR4/MyD88-induced CD11b+Gr-1intF4/80+ non-migratory myeloid cells suppress Th2 effector function in the lung. Mucosal Immunol. 2010;3:578-93. (FC)
11. Duewell P, Kisser U, Heckelsmiller K, Hoves S, Stoitzner P, Koernig S, et al. ISCOMATRIX adjuvant combines immune activation with antigen delivery to dendritic cells in vivo leading to effective cross-priming of CD8+ T cells. J Immunol. 2011;187:55-63. (FC)
12. Szyszko EA, Skarstein K, Jonsson R, Brokstad KA. Distinct phenotypes of plasma cells in spleen and bone marrow of autoimmune NOD.B10.H2b mice. Autoimmunity. 2011;44:415-26. (FC)
13. Khare A, Krishnamoorthy N, Oriss TB, Fei M, Ray P, Ray A. Cutting edge: inhaled antigen upregulates retinaldehyde dehydrogenase in lung CD103+ but not plasmacytoid dendritic cells to induce Foxp3 de novo in CD4+ T cells and promote airway tolerance. J Immunol. 2013;191:25-29. (FC)

Spectral Red® is a registered trademark of Southern Biotechnology Associates, Inc.

Spectral Red® is a PE/CY5 tandem conjugate

Cy® is a registered trademark of GE Healthcare

細胞免疫專家極力推薦SouthernBiotech高品質抗體

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質抗體。物種種類齊全，包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質（FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）各種螢光標誌、另具酵素（HRP、AP）、或未標誌及Low Endotoxin/Azide Free（LE/AF）等標定。

1895-01	Rat Anti-Mouse MHC Class II-UNLB	NIMR-4	0.5 mg
1895-02	Rat Anti-Mouse MHC Class II-FITC	NIMR-4	0.5 mg
1895-08	Rat Anti-Mouse MHC Class II-BIOT	NIMR-4	0.5 mg
1895-09	Rat Anti-Mouse MHC Class II-PE	NIMR-4	0.1 mg
1895-09L	Rat Anti-Mouse MHC Class II-PE	NIMR-4	0.2 mg
1895-11	Rat Anti-Mouse MHC Class II-APC	NIMR-4	0.1 mg
1895-13	Rat Anti-Mouse MHC Class II-SPRD	NIMR-4	0.1 mg
1895-14	Rat Anti-Mouse MHC Class II-LE/AF	NIMR-4	0.5 mg

Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |