EZCell™鈣染色試劑盒 | 貨號K432-50

EZCell™ Calcium Detection Kit (Cell-Based) Sensitive Cell-Based Assay for determination of intracellular calcium

產品描述：Calcium鈣對所有生物都是必不可少的。Calcium sequestration鈣螯合和 release 釋放到cytoplasm細胞質中作為許多細胞過程的信號。鈣作為重要的 second messenger第二信使，參與許多physiological processes 生理過程，如神經衝動傳遞nerve impulse transmission、muscle contraction肌肉收縮等。細胞內鈣主要儲存在mitochondria線粒體和 endoplasmic reticulum內質網中，其在細胞質中的濃度受到嚴格調節。Dysfunction  of calcium homeostasis 鈣穩態功能障礙導致oxidative stress氧化應激、cell death細胞死亡和Alzheimer’s disease阿爾茨海默病的發病機制。 BioVision開發了一種EZCell™鈣染色試劑盒，其中含有可滲透膜的細胞內鈣探針。細胞進入後，鈣探針被細胞內酯酶水解intracellular esterases，使其膜不可滲透。裂解的鈣探針與細胞內產生鈣的螢光結合。螢光強度與細胞內鈣水平成正比。還提供了陽性對照試劑，其用作實驗對照，其提高細胞內鈣水平，導致鈣染色增加。這種易於使用的非放射性試劑盒可通過在培養細胞中使用螢光顯微鏡和流式細胞儀研究細胞水平的鈣調節。

Cat # +Size	K432-50
Size	50 assays
Kit Summary	• Detection method- Fluorescence Microscopy/ Flow Cytometer (FL1) • Species reactivity- Mammalian • Applications- The assay kit can selectively detect intracellular calcium content in suspension and/or adherent cells
Detection Method	Fluorescence Microscopy/ Flow Cytometer (FL1)
Species Reactivity	Eukaryotes
Applications	The assay kit can selectively detect intracellular calcium content in suspension and/or adherent cells
Features & Benefits	• Simple procedure; takes ~ 2 hours • Fast and convenient • The assay is sensitive, stable, specific and high-throughput adaptable • Applications- the kit can visualize intracellular calcium content in suspension and/or adherent cells
Kit Components	• Assay Buffer (50X) • Calcium Probe • Experimental Control (2000X)
Storage Conditions	-20°C
Shipping Conditions	Gel Pack