MTT細胞增殖分析試劑盒 | 貨號K299-1000  (ab211091)

Non-radioactive and high-throughput method to measure cell proliferation/viability/cytotoxicity

活細胞數量的定量是細胞生物學研究中不可或缺的工具。 BioVision的MTT細胞增殖分析試劑盒是一種靈敏的方法，用於定量增殖和細胞毒性分析中的活細胞。 該方法基於水溶性MTT（溴化3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑）化合物向不溶性 formazan product產物的轉化。 具有活性代謝的活細胞將MTT轉化為 formazan，然而，死細胞喪失了這種能力。 因此，顏色形成僅作為活細胞的有用且方便的標記。 測得的吸光度（590nm）與活細胞數成比例。 該檢測試劑盒為提供了easy-to-use，non-radioactive非放射性和high-throughput method 高通量的方法。評估細胞之 cell proliferation細胞增殖、 cell viability chemotaxis趨化性、cytotoxicity細胞毒性和 apoptosis細胞凋亡。

• Assay type: Quantitative
• Detection method: Colorimetric
• Platform: Microplate reader
• Assay time: 3 hr 15 min
• Sample type: Adherent cells, Suspension cells

Cat # +Size	K299-1000
Size	1000 assays
Detection Method	Absorbance (490 nm)
Applications	Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Assessment of physiological mediators that inhibit cell growth.
Features & Benefits	• Non-radioactive • High-throughput • The entire assay can be performed directly in a 96-well plate and does not require washing/harvesting/or solubilization steps.
Kit Components	• MTT Reagent • MTT Solvent