前脂肪細胞分離試劑盒 | 貨號K583-5

Lipolysis (Adipocyte) Colorimetric/Fluorometric Assay Kit Only kit on the market to isolate adipocytes from adipose tissue & measure lipolysis

產品描述：脂肪分解是甘油三酯細胞內水解成甘油和游離脂肪酸，然後釋放到血流或培養基中。脂解作用基本上在所有細胞中發生，但在白色和棕色脂肪組織中最豐富。脂肪分解的缺陷導致細胞內脂質積累增加，導致異常的細胞生理學，高脂血症和胰島素抵抗。激素或兒茶酚胺可誘導脂肪分解，其與β-腎上腺素能受體結合，導致腺苷酸環化酶活化，從ATP形成cAMP。 cAMP作為激活激素敏感性脂肪酶的第二信使，其水解甘油三酯。該途徑受胰島素抑制。 BioVision的脂肪細胞脂肪分解比色/螢光檢測試劑盒是監測脂肪分解的簡便方法。該試劑盒提供了從多達5g的小鼠或大鼠脂肪組織中分離脂肪細胞所需的所有試劑，並在誘導脂解後測量來自原代脂肪細胞的甘油釋放。它還包括合成的兒茶酚胺，異丙腎上腺素，以刺激cAMP介導的途徑。信號強度與存在的甘油量成正比。該測定試劑盒可以在比色測定中檢測小於200pmol的甘油，螢光測定中檢測小於20pmol。

Cat # +Size	K581-5
Size	For 5 g Tissue
Detection Method	Absorbance (OD 570 nm) and Fluorescence (Ex/Em = 535/587 nm)
Applications	Isolation of adipocytes from rat/mouse tissue Measurement of lipolysis by adipocytes Screening compounds that influence lipolysis, mechanistic studies and studies on metabolic dysfunction
Features & Benefits	• Simple, rapid & convenient method to isolate adipocytes from adipose tissue & measure lipolysis. • The assay kit can detect less than 200 pmol glycerol in the colorimetric assay and less than 20 pmol in the fluorometric assay.
Kit Components	• Collagenase (0.2%) • Collagenase Stop Buffer • Adipocyte Wash Buffer • Adipocyte Lipolysis Buffer • Glycerol Assay Buffer • Glycerol Probe (in DMSO, anhydrous) • Glycerol Enzyme Mix (Lyophilized) • Glycerol Standard (100 mM) • Isoproterenol (10 mM) • Cell strainer (100 µm)
Storage Conditions	-20°C
Shipping Conditions	Gel Pack