酸性磷酸酶檢測試劑盒 | 貨號K411-500 (ABCAM ab83367) 

Acid Phosphatase Activity Colorimetric Assay Kit

產品描述：酸性磷酸酶Acid phosphatases（AP）在酸性條件下使磷酸酯基團的磷酸酯基團去磷酸化。 在不同器官中發現不同的acid phosphatase isozymes酸性磷酸酶同工酶，其血清水平用為相應器官中疾病的診斷。 升高的前列腺酸性磷酸酶水平可能表明存在前列腺癌，並且Tartrate-Resistant Acid Phosphatase酒石酸抗性酸性磷酸酶水平升高可能表明bone disease骨病。 BioVision的酸性磷酸酶檢測試劑盒是一種高靈敏度，簡單，直接和HTS就緒的比色檢測方法，用於測量血清和其他樣品中的AP活性。 它適用於research研究和drug discovery藥物發現。 該試劑盒使用磷酸對硝基苯酯（pNPP）作為磷酸酶底物，當AP去磷酸化時，其變黃（λmax= 405nm）。 該試劑盒可以檢測樣品中的μU酸性磷酸酶活性。

引用文獻

Wei Wang. Isofraxidin Inhibits Receptor Activator of Nuclear Factor-?B Ligand–Induced Osteoclastogenesis in Bone Marrow–Derived Macrophages Isolated from Sprague–Dawley Rats by Regulating NF-?B/NFATc1 and Akt/NFATc1 Signaling Pathways. Cell Transplant. Feb 2021. 33573387.

• Product name

  Acid Phosphatase Assay Kit (Colorimetric)
  See all Acid phosphatase kits

• Detection method

  Colorimetric

• Sample type

  Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media

• Assay type

  Enzyme activity

• Sensitivity

  > 0.001 mM

• Range

  0.001 mM – 1 mM

• Assay time

  1h 10m

• Product overview

  Acid Phosphatase Assay Kit ab83367 is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure acid phosphatase activity in serum and other samples.
  The acid phosphatase assay protocol uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (absorbance max 405 nm) when dephosphorylated by acid phosphatase.
  Acid phosphatase assay protocol summary:
  – add samples and standards to wells
  – add pNPP to samples
  – add acid phosphatase enzyme to standards
  – incubate for 60 min
  – add stop solution
  – analyze with microplate reader

• Notes

  This product is manufactured by BioVision, an Abcam company and was previously called K411 Acid Phosphatase Activity Colorimetric Assay Kit. K411-500 is the same size as the 500 test size of ab83367.

  Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease.

  pNPP Assays

  pNPP assays are a class of phosphatase assays using p-nitrophenyl phosphate (pNPP) as a phosphatase substrate. They can be used to measure alkaline phosphatase, neutral phosphatase, and acid phosphatase activity. These different types of phosphatases are active respectively in alkaline, neutral, and acid assay buffers. pNPP assays include Alkaline Phosphatase Assay Kit ab83369 and Acid Phosphatase Assay Kit ab83367.