Annexin V-FITC細胞凋亡檢測 | ApoScreen® Annexin V Apoptosis Kit-FITC SBA貨號10010-02

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Annexin V-FITC細胞凋亡檢測 | 貨號10010-02 100個反應
ApoScreen® Annexin V Apoptosis Kit-FITC
細胞凋亡產品介紹: 細胞凋亡的早期階段,大多數細胞將磷脂醯絲氨酸(PS)從質膜的內表面轉移至細胞表面。膜聯蛋白V (Annexin V)是一種Ca離子依賴性磷脂結合蛋白,可以與磷脂醯絲氨酸phosphatidylserine (PS) 特異結合。套組內包括有Annexin V-FITC及碘化丙啶(PI)。套組內包括有Annexin V-FITC及碘化丙啶(PI)。應用FITC結合標記的Annexin V通過流式細胞儀或螢光顯微鏡可以檢測到細胞凋亡的發生。結合細胞核染劑PI同時可偵測偵測細胞凋亡現象及其凋亡分期。同時評估細胞凋亡與細胞壞死機制。當細胞用膜聯蛋白V-FITC和碘化丙啶(PI)雙重染色時,可以觀察到三種不同的細胞群: 活細胞不被Annexin V-FITC 或 PI染色 、necrotic cells壞死細胞同時被兩種試劑染色、凋亡細胞僅可被膜聯蛋白V-FITC染色通過流式細胞儀488nm激發進行分析。
Day old BALB/c mouse splenocytes were stained with ApoScreen® Annexin V Apoptosis Kit-FITC (SB Cat. No. 10010-02).
Kit Components | Annexin V-FITC – 100 tests in 1.0 mL Propidium Iodide (PI) – 100 tests in 1.0 mL Annexin V Binding Buffer 10X – 7 x 1.5 mL |
Buffer Formulation | Annexin V-FITC and PI are supplied in Annexin V Binding Buffer |
Storage & Handling | Store at 2-8°C Please refer to product specific SDS |
Applications | Flow Cytometry – Quality tested 1-7, 9-34 Immunocytochemistry – Reported in literature 6,8,35 |
Recommended Dilutions | Please refer to product specific Technical Bulletin |
細胞免疫專家極力推薦SouthernBiotech高品質二級抗體
美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全,包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質(FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)各種螢光標誌、另具酵素(HRP、AP)、或未標誌及Low Endotoxin/Azide Free(LE/AF)等標定。
Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |
Cat. No. | Product Description | Size |
10010-02 | ApoScreen® Annexin V Apoptosis Kit-FITC | 100 tests |
10010-09 | ApoScreen® Annexin V Apoptosis Kit-PE | 100 tests |
10039-01 | ApoScreen® Annexin V-UNLB | 0.1 mg |
10039-02 | ApoScreen® Annexin V-FITC | 100 tests |
10039-08 | ApoScreen® Annexin V-BIOT | 100 tests |
10040-01 | ApoScreen® Annexin V-UNLB | 0.1 mg |
10040-02 | ApoScreen® Annexin V-FITC | 100 tests |
10040-08 | ApoScreen® Annexin V-BIOT | 100 tests |
10040-09 | ApoScreen® Annexin V-PE | 100 tests |
10040-11 | ApoScreen® Annexin V-APC | 100 tests |
10040-15 | ApoScreen® Annexin V-CY5 | 100 tests |
10042-01 | ApoScreen® 7-Aminoactinomycin D (7-AAD) | 100 tests |
10044-01 | ApoScreen® Propidium Iodide (PI) | 100 tests |
10045-01 | ApoScreen® Annexin V Binding Buffer 10X | 50 mL |
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