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Annexin V-PE細胞凋亡檢測 | 膜聯蛋白V-PE/7-ADD雙染檢測SBA 貨號10010-09

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Annexin V-PE細胞凋亡檢測操作手冊

特點

Annexin V-PE細胞凋亡檢測  | 貨號10010-09        100個反應

ApoScreen® Annexin V Apoptosis Kit-PE    

細胞凋亡產品介紹: 細胞凋亡的早期階段,大多數細胞將磷脂醯絲氨酸(PS)從質膜的內表面轉移至細胞表面。膜聯蛋白V (Annexin V)是一種Ca離子依賴性磷脂結合蛋白,可以與磷脂醯絲氨酸phosphatidylserine (PS) 特異結合。套組內包括有Annexin V-PE及7-AAD。應用R-藻紅蛋白結合標記的Annexin V通過流式細胞儀或螢光顯微鏡可以檢測到細胞凋亡的發生。結合細胞核染劑7-氨基放線菌素D(7-AAD)同時可偵測偵測細胞凋亡現象及其凋亡分期。同時評估細胞凋亡與細胞壞死機制。當細胞用膜聯蛋白V-PE和7-AAD雙重染色時,可以觀察到三種不同的細胞群: 活細胞不被Annexin V-PE 或 7-AAD染色 、necrotic cells壞死細胞同時被兩種試劑染色、凋亡細胞僅可被膜聯蛋白V-PE染色通過流式細胞儀488nm激發進行分析。

Day old BALB/c mouse splenocytes were stained with ApoScreen® Annexin V Apoptosis Kit-PE (SB Cat. No. 10010-09).

Kit Components Annexin V-PE – 100 tests in 1.0 mL
7-Aminoactinomycin D (7-AAD) – 100 tests in 1.0 mL
Annexin V Binding Buffer 10X – 7 x 1.5 mL
Buffer Formulation Annexin V-PE is supplied in PBS/NaN3 and a stabilizing agent
7-AAD is supplied in PBS/NaN3
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications Flow Cytometry – Quality tested 1-35
Recommended Dilutions Please refer to product specific Technical Bulletin

細胞免疫專家極力推薦SouthernBiotech高品質二級抗體

美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全,包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質(FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)各種螢光標誌、另具酵素(HRP、AP)、或未標誌及Low Endotoxin/Azide Free(LE/AF)等標定。

Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |

Cat. No. Product Description Size
10010-02 ApoScreen® Annexin V Apoptosis Kit-FITC 100 tests
10010-09 ApoScreen® Annexin V Apoptosis Kit-PE 100 tests
10039-01 ApoScreen® Annexin V-UNLB 0.1 mg
10039-02 ApoScreen® Annexin V-FITC 100 tests
10039-08 ApoScreen® Annexin V-BIOT 100 tests
10040-01 ApoScreen® Annexin V-UNLB 0.1 mg
10040-02 ApoScreen® Annexin V-FITC 100 tests
10040-08 ApoScreen® Annexin V-BIOT 100 tests
10040-09 ApoScreen® Annexin V-PE 100 tests
10040-11 ApoScreen® Annexin V-APC 100 tests
10040-15 ApoScreen® Annexin V-CY5 100 tests
10042-01 ApoScreen® 7-Aminoactinomycin D (7-AAD) 100 tests
10044-01 ApoScreen® Propidium Iodide (PI) 100 tests
10045-01 ApoScreen® Annexin V Binding Buffer 10X 50 mL

References

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ApoScreen® is a registered trademark of SouthernBiotechnology Associates, Inc.

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