Annexin V-PE細胞凋亡檢測  | 貨號10010-09        100個反應

ApoScreen® Annexin V Apoptosis Kit-PE    

細胞凋亡產品介紹: 細胞凋亡的早期階段，大多數細胞將磷脂醯絲氨酸（PS）從質膜的內表面轉移至細胞表面。膜聯蛋白V (Annexin V)是一種Ca離子依賴性磷脂結合蛋白，可以與磷脂醯絲氨酸phosphatidylserine (PS) 特異結合。套組內包括有Annexin V-PE及7-AAD。應用R-藻紅蛋白結合標記的Annexin V通過流式細胞儀或螢光顯微鏡可以檢測到細胞凋亡的發生。結合細胞核染劑7-氨基放線菌素D（7-AAD）同時可偵測偵測細胞凋亡現象及其凋亡分期。同時評估細胞凋亡與細胞壞死機制。當細胞用膜聯蛋白V-PE和7-AAD雙重染色時，可以觀察到三種不同的細胞群: 活細胞不被Annexin V-PE 或 7-AAD染色 、necrotic cells壞死細胞同時被兩種試劑染色、凋亡細胞僅可被膜聯蛋白V-PE染色通過流式細胞儀488nm激發進行分析。

Day old BALB/c mouse splenocytes were stained with ApoScreen® Annexin V Apoptosis Kit-PE (SB Cat. No. 10010-09).

Kit Components	Annexin V-PE – 100 tests in 1.0 mL 7-Aminoactinomycin D (7-AAD) – 100 tests in 1.0 mL Annexin V Binding Buffer 10X – 7 x 1.5 mL
Buffer Formulation	Annexin V-PE is supplied in PBS/NaN3 and a stabilizing agent 7-AAD is supplied in PBS/NaN3
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Flow Cytometry – Quality tested 1-35
Recommended Dilutions	Please refer to product specific Technical Bulletin

細胞免疫專家極力推薦SouthernBiotech高品質二級抗體

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全，包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質（FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）各種螢光標誌、另具酵素（HRP、AP）、或未標誌及Low Endotoxin/Azide Free（LE/AF）等標定。

Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |

Cat. No.	Product Description	Size
10010-02	ApoScreen® Annexin V Apoptosis Kit-FITC	100 tests
10010-09	ApoScreen® Annexin V Apoptosis Kit-PE	100 tests
10039-01	ApoScreen® Annexin V-UNLB	0.1 mg
10039-02	ApoScreen® Annexin V-FITC	100 tests
10039-08	ApoScreen® Annexin V-BIOT	100 tests
10040-01	ApoScreen® Annexin V-UNLB	0.1 mg
10040-02	ApoScreen® Annexin V-FITC	100 tests
10040-08	ApoScreen® Annexin V-BIOT	100 tests
10040-09	ApoScreen® Annexin V-PE	100 tests
10040-11	ApoScreen® Annexin V-APC	100 tests
10040-15	ApoScreen® Annexin V-CY5	100 tests
10042-01	ApoScreen® 7-Aminoactinomycin D (7-AAD)	100 tests
10044-01	ApoScreen® Propidium Iodide (PI)	100 tests
10045-01	ApoScreen® Annexin V Binding Buffer 10X	50 mL

References

1. Schwartzenberg S, Deutsch V, Maysel-Auslender S, Kissil S, Keren G, George J. Circulating apoptotic progenitor cells: a novel biomarker in patients with acute coronary syndromes. Arterioscler Thromb Vasc Biol. 2007;27:e27-31. (FC)
2. Ben-Shoshan J, Maysel-Auslender S, Mor A, Keren G, George J. Hypoxia controls CD4+CD25+ regulatory T-cell homeostasis via hypoxia-inducible factor-1α. Eur J Immunol. 2008;38:2412-8. (FC)
3. Qian C, Yan W, Zhang J, Shi L, Qian J, Fu Z, et al. Notch1 induces enhanced expression of Δ-like-1 in the U251MG glioma cell line. Int J Mol Med. 2009;24:445-51. (FC)
4. Hsieh C, Hernández-Ledesma B, de Lumen BO. Lunasin, a novel seed peptide, sensitizes human breast cancer MDA-MB-231 cells to aspirin-arrested cell cycle and induced apoptosis. Chem Biol Interact. 2010;186:127-34. (FC)
5. Yan Y, Zheng M, Chen Z, Yu X, Yang X, Ding Z, et al. Studies on preparation and photodynamic mechanism of chlorin P6-13,15-N-(cyclohexyl)cycloimide (Chlorin-H) and its antitumor effect for photodynamic therapy in vitro and in vivo. Bioorg Med Chem. 2010;18:6282-91. (FC)
6. Li W, Li Y, Guo J, Liu Y, Yang X, Ge H, et al. Hepatocytic precursor (stem-like) WB-F344 cells reduce tumorigenicity of hepatoma CBRH-7919 cells via TGF-β/Smad pathway. Oncol Rep. 2010;23:1601-7. (FC)
7. Jung C, Rafnsson A, Shemyakin A, Böhm F, Pernow J. Different subpopulations of endothelial progenitor cells and circulating apoptotic progenitor cells in patients with vascular disease and diabetes. Int J Cardiol. 2010;143:368-72. (FC)
8. Yan F, Tan X, Geng Y, Ju H, Gao Y, Zhu M. Inhibition effect of siRNA-downregulated UHRF1 on breast cancer growth. Cancer Biother Radiopharm. 2011;2:183-9. (FC)
9. Yao K, Wang W, Zhu Y, Jin C, Shentu X, Jiang J, et al. A novel GJA3 mutation associated with congenital nuclear pulverulent and posterior polar cataract in a Chinese family. Hum Mutat. 2011;32:1367-70. (FC)
10. Hsieh C, Hernández-Ledesma B, de Lumen BO. Cell proliferation inhibitory and apoptosis-inducing properties of anacardic acid and lunasin in human breast cancer MDA-MB-231 cells. Food Chem. 2011;125:630-6. (FC)
11. Shi X, Su S, Long J, Mei B, Chen Y. MicroRNA-191 targets N-deacetylase/N-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803. Acta Biochim Biophys Sin. 2011;43:849-56. (FC)
12. Li X, Guo Q, Wang X, Xue B, Sun L, Meng Q, et al. LRP16 gene protects mouse insulinoma MIN6 cells against fatty acid-induced apoptosis through Akt/FoxO1 signaling. Chin Med J. 2012;125:1695-1702. (FC)
13. Zhang Y, Du X, Wang C, Lin D, Ruan X, Feng Y, et al. Reciprocal activation between PLK1 and Stat3 contributes to survival and proliferation of esophageal cancer cells. Gastroenterology. 2012;142:521-30. (FC)
14. Ma Y, Jiang J, Wang L, Nie H, Xia W, Liu J, et al. CD38 is a key enzyme for the survival of mouse microglial BV2 cells. Biochem Biophys Res Commun. 2012;418:714-9. (FC)
15. He X, Nie H, Hong Y, Sheng C, Xia W, Ying W. SIRT2 activity is required for the survival of C6 glioma cells. Biochem Biophys Res Commun. 2012;417:468-72. (FC)
16. Choi YJ, Li X, Hydbring P, Sanda T, Stefano J, Christie AL, et al. The requirement for cyclin D function in tumor maintenance. Cancer Cell. 2012;22:438-51. (FC)
17. He X, Shao Y, Li X, Xu W, Chen G, Sun H, et al. Downregulation of miR-101 in gastric cancer correlates with cyclooxygenase-2 overexpression and tumor growth. FEBS J. 2012;279:4201-12. (FC)
18. Xu R, Lin F, He J, Jin L, Zhang J, Fu J, et al. Complement 5a stimulates hepatic stellate cells in vitro, and is increased in the plasma of patients with chronic hepatitis B. Immunology. 2012;138:228-34. (FC)
19. Xie W, Xie H, Liu F, Li W, Dan J, Mei Y, et al. Propranolol induces apoptosis of human umbilical vein endothelial cells through downregulation of CD147. Br J Dermatol. 2013;168:739-48. (FC)
20. Jung C, Rafnsson A, Brismar K, Pernow J. Endothelial progenitor cells in relation to endothelin-1 and endothelin receptor blockade: a randomized, controlled trial. Int J Cardiol. 2013;168:1017-22. (FC)
21. Wang Y, Guo R, Guo R, Sheng W, Yin L. MicroRNA-182 promotes cell growth, invasion, and chemoresistance by targeting programmed cell death 4 (PDCD4) in human ovarian carcinomas. J Cell Biochem. 2013;114:1464-73. (FC)
22. Li Y, Nie H, Wu D, Zhang J, Wei X, Ying W. Poly(ADP-ribose) polymerase mediates both cell death and ATP decreases in SIRT2 inhibitor AGK2-treated microglial BV2 cells. Neurosci Lett. 2013;544:36-40. (FC)
23. Humphries LA, Godbersen JC, Danilova OV, Kaur P, Christensen BC, Danilov AV. Pro-apoptotic TP53 homolog TAp63 is repressed via epigenetic silencing and B-cell receptor signalling in chronic lymphocytic leukaemia. Br J Haematol. 2013;163:590-602. (FC)
24. Zhu J, Hong D, Song Y, Sun L, Wang Z, Wang J. Suppression of cellular apoptosis susceptibility (CSE1L) inhibits proliferation and induces apoptosis in colorectal cancer cells. Asian Pac J Cancer Prev. 2013;14:1017-21. (FC)
25. Hong Y, Nie H, Wu D, Wei X, Ding X, Ying W. NAD+ treatment prevents rotenone-induced apoptosis and necrosis of differentiated PC12 cells. Neurosci Lett. 2014;560:46-50. (FC)
26. Chen X, Chen Y, Lan J, Shen Z. MicroRNA-370 suppresses proliferation and promotes endometrioid ovarian cancer chemosensitivity to cDDP by negatively regulating ENG. Cancer Lett. 2014;353:201-10. (FC)
27. Wang K, Ma J, Zhang F, Yu M, Xue J, Zhao J. MicroRNA-378 inhibits cell growth and enhances L-OHP-induced apoptosis in human colorectal cancer. IUBMB Life. 2014;66:645-54. (FC)
28. Godbersen JC, Humphries LA, Danilova OV, Kebbekus PE, Brown JR, Eastman A, et al. The Nedd8-activating enzyme inhibitor MLN4924 thwarts microenvironment-driven NF-κB activation and induces apoptosis in chronic lymphocytic leukemia B cells. Clin Cancer Res. 2014;20:1576-89. (FC)
29. Nie H, Li Y, Wang C, Chen X, Liu B, Wu D, et al. SIRT2 plays a key role in both cell cycle regulation and cell survival of BV2 microglia. Int J Physiol Pathophysiol Pharmacol. 2014;6:166-71. (FC)
30. Feng G, Zhang J, Zeng Q, Yu X, Zhang Z, Lv S, et al. Interleukin-21 mediates hepatitis B virus-associated liver cirrhosis by activating hepatic stellate cells. Hepatol Res. 2014;44:E198-205. (FC)
31. Wang C, Chen H, Zhang J, Hong Y, Ding X, Ying W. Malate-aspartate shuttle mediates the intracellular ATP levels, antioxidation capacity and survival of differentiated PC12 cells. Int J Physiol Pathophysiol Pharmacol. 2014;6:109-14. (FC)
32. Yao Y, Ma J, Wang P, Xue Y, Li Z, Zhao L, et al. miR-101 acts as a tumor suppressor by targeting Kruppel-like factor 6 in glioblastoma stem cells. CNS Neurosci Ther. 2015;21:40-51. (FC)
33. Wang C, Zhang J, Zhang M, Chen H, Ying W. Aralar plays a significant role in maintaining the survival and mitochondrial membrane potential of BV2 microglia. Int J Physiol Pathophysiol Pharmacol. 2015;7:107-114. (FC)
34. Paiva C, Godbersen JC, Berger A, Brown JR, Danilov AV. Targeting neddylation induces DNA damage and checkpoint activation and sensitizes chronic lymphocytic leukemia B cells to alkylating agents. Cell Death Dis. 2015;6:e1807. (FC)
35. Johnson RM, Vu NT, Griffin BP, Gentry AE, Archer KJ, Chalfant CE, et al. The alternative splicing of cytoplasmic polyadenylation element binding protein 2 drives anoikis resistance and the metastasis of triple negative breast cancer. J Biol Chem. 2015;290:25717-27. (FC)

ApoScreen® is a registered trademark of SouthernBiotechnology Associates, Inc.