溴脫氧尿苷（BrdU）胸苷類似物 | Bromodeoxyuridine (BrdU) 貨號X2834
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溴脫氧尿苷（BrdU）Bromodeoxyuridine (BrdU) | X2834
溴脫氧尿苷（BrdU）是胸苷類似物，用於細胞增殖研究。 培養中的BrdU在DNA合成過程中被整合到DNA中。 通過流式細胞術或免疫組織化學膜透化後，可以通過抗BrdU特異性抗體檢測BrdU的細胞摻入。 BrdU的分子量為307.1。BrdU免疫染色檢測增殖細胞之前，必須用BrdU標記細胞或組織。 標記可以在體外進行細胞培養，也可以在體內進行實驗動物。
Catalogue number: X2834
| Product Type | Reagent |
| Units | 25 mg |
Product Form
Unconjugated
Background
Bromo-deoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into the DNA during DNA synthesis. Cellular incorporation of BrdU can be detected by anti-BrdU specific antibodies following membrane permeabilization by flow cytometry or immunohistochemistry. The molecular weight of BrdU is 307.1.
Concentration
See vial for concentration
Formulation
Provided as 5 x 5 mg in sterile filtered solution in phosphate buffered saline
Functional Analysis
Immunohistochemistry
Applications
Prior to BrdU immunostaining to detect proliferating cells it is necessary to label the cells or tissue with BrdU. Labeling can either be done in vitro for cell cultures or performed in vivo for experimental animals. Both protocols are provided.
Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Ship Conditions
Ship at ambient temperature, freeze upon arrival
Product Stability
Products are stable for one year from purchase when stored properly
| Code | Name | Product Type | Clone |
|---|---|---|---|
| ABrdU | Chicken anti Bromodeoxyuridine (BrdU) | Primary Antibodies | |
| X2840K | BrdU Fluorescent Immunohistochemistry Kit – FL549 | Immunofluorescence Kit | |
| X2839K | BrdU Fluorescent Immunohistochemistry Kit – FL488 | Immunofluorescence Kit | |
| X2834 | Bromodeoxyuridine (BrdU) | Reagent | |
| X2743 | BrdU Slides | Slides | |
| X1623K3 | BrdU Chemiluminescent Cell Proliferation Assay Kit | Assay Kit | |
| X1623K2 | BrdU Chemiluminescent Cell Proliferation Assay Kit | Assay Kit | |
| X1623K1 | BrdU Chemiluminescent Cell Proliferation Assay Kit | Assay Kit | |
| X1545K | BrdU Immunohistochemistry Kit | Immunohistochemistry Kit | |
| X1543M | Mouse anti BrdU (bromodeoxyuridine), conjugated with FITC | Monoclonal Antibody | MoBU-1 |
| X1327K3 | BrdU Cell Proliferation Assay Kit | Assay Kit | |
| X1327K2 | BrdU Cell Proliferation Assay Kit | Assay Kit | |
| X1327K1 | BrdU Cell Proliferation Assay Kit | Assay Kit | |
| X1028 | Mouse anti BrdU (bromodeoxyuridine) | Monoclonal Antibody | MoBU |
| MUB0200P | Mouse anti Bromodeoxyuridine / BrdU | Primary Antibodies | IIB5 |
| MUB0200L2 | Mouse anti Bromodeoxyuridine/BrdU, conjugated to Horse Radish Peroxidase | Primary Antibodies | IIB5 |
| A205P | Sheep anti BrdU (bromodeoxyuridine) | Polyclonal Antibody | |
| MUB1200P | Mouse anti Mitotic cells | Primary Antibodies | 8B3G |