葡萄糖-6-磷酸比色測定試劑盒 | Glucose-6-Phosphate Colorimetric Assay Kit貨號K657-100
| 代理廠牌： |  |
| 原廠連結： | |
| 相關下載： |
特點
Glucose-6-Phosphate Colorimetric Assay Kit
產品描述：葡萄糖-6-磷酸（G6P）是葡萄糖進入細胞的關鍵糖中間體，然後進入代謝途徑或儲存。 G6P可以進入糖酵解途徑，磷酸戊糖分流或作為糖原或澱粉貯存。 G6P被其脫氫酶利用以產生NADPH形式的還原當量。 這在G6PDH缺陷導致溶血性貧血的紅細胞中尤為重要。 BioVision的葡萄糖-6-磷酸測定試劑盒是一種簡單，靈敏且快速的量化各種樣品中G6P的方法。 在該測定中，葡萄糖-6-磷酸被產生一種產物而被氧化，該產物被用於將幾乎無色的探針轉化成具有450nm吸光度的強烈著色的產物。 葡萄糖-6-磷酸測定試劑盒可檢測1至30納摩爾範圍內的G6P，檢測靈敏度約為10μMG6P。
| Cat # +Size | K657-100 |
| Size | 100 assays |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Mammalian |
| Applications | The Glucose-6-phosphate Assay Kit can detect G6P in the range of 1 to 30 nmoles with detection sensitivity ~10 µM of G6P. |
| Features & Benefits | • Simple procedure; takes ~ 40 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Glucose-6-Phosphate (G6P) |
| Kit Components | • G6P Assay Buffer • G6P Enzyme Mix • G6P Substrate Mix • G6PStandard (10 µmol) |
| Storage Conditions | -20°C |
引用文獻
Guang-Zhi Jin, et al., ( 2018) Phosphoglucomutase 1 inhibits hepatocellular carcinoma progression by regulating glucose trafficking, PLoS Biology, Oct. 2018, 30335765
Shearer, J. et. al. Exercise training does not correct abnormal cardiac glycogen accumulation in the db/db mouse model of type 2 diabetes. Am J Physiol Endocrinol Metab, Jul 2011; 301: E31 – E39.