NFkB p65 Antibody | 貨號3038-100

產品描述：NFkB是由50kDa DNA結合亞基（p50）和65kDa反式激活亞基（p65 / RelA）組成的異二聚體。 這兩個亞基都顯示與原癌基因c-Rel的序列同源性。 p50具有稱為p49 / p52的同種型，並且這兩種蛋白都來源於前體蛋白p105和p100的氨基末端。 p50 / p65異二聚體作為與其抑製劑IkB的複合物以無活性形式保留在胞質溶膠中。 通過多種刺激如脂多醣（LPS），促炎細胞因子（IL-1和TNF等）和病毒感染刺激細胞後，IkB被蛋白體磷酸化並降解。 有活性的NFkB異源二聚體易位至細胞核並誘導基因表達。

Antibody Target	NFkB p65
Alternate Name	RELA, NFKB3, MGC131774
Host	Rabbit
Antibody Type	Polyclonal
Isotype	Rabbit IgG
Immunogen	Synthetic peptide surrounding amino acid 274 of mouse NFkB p65
Accession #	NP_033071
Gene ID	19697
Appearance	Colorless liquid
Concentration	0.2 mg/ml
Formulation	100 µg (0.2 mg/ml) affinity purified rabbit anti-NFkB polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 25% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification	Affinity purified
Species Reactivity	Human, mouse, rat
Application	Western blot, Immunohistochemistry
Application & Usage	Western blotting (0.5-4 µg/ml), immunocytochemistry (10-20µg/ml). However, the optimal conditions should be determined individually. The antibody detects primarily NFkB p65 in samples of human, mouse, and rat origins.
Handling	The antibody solution should be gently mixed before use.