丙酮酸脫氫酶（PDH）活性檢測 | Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit貨號K679-100

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丙酮酸脫氫酶（PDH）活性比色測定試劑盒 | 貨號K679-100

Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit

產品描述：丙酮酸脫氫酶（Pyruvate Dehydrogenase, PDH）（EC 1.2.4.1）在碳水化合物代謝 (carbohydrate metabolism) 中具有重要作用。它與二氫硫辛酰轉乙酰酶（dihydrolipoyl transacetylase, E2）和二氫硫辛酰脫氫酶（ dihydrolipoyl dehydrogenase, E3）形成了一個特徵明確的酶複合物。 PDH在NAD和CoA存在下將丙酮酸轉化為乙酰-CoA，並將glycolysis 糖酵解與 citric acid cycle檸檬酸循環聯繫起來。 PDH活性受ATP / ADP，NADH / NAD或乙酰輔酶A /輔酶A的高細胞內比率的抑制。在人類中，PDH缺乏會降低線粒體功能，並與神經退行性疾病 ( neurodegenerative diseases)有關。 PDH缺陷是X連鎖的;它導致2種形式的異常：代謝形式（lactic acidosis乳酸酸中毒）和神經形式（seizure癲癇發作和/或neuropathological spasm神經病理性痙攣）。最近的研究表明，PDH是致癌基因誘導的衰老的靶標; PDH的激活增強丙酮酸利用並增加呼吸和氧化還原應激。 BioVision的PDH檢測試劑盒為監測各種樣品中的PDH活性提供了一種快速簡單的方法。在該測定中，PDH將丙酮酸轉化成中間體，其將顯色劑還原成在450nm處具有強吸光度的有色產物。該檢測方法簡單，靈敏，可在各種樣品中檢測到低於0.1 mU的丙酮酸脫氫酶活性。

Cat # +Size	K679-100
Size	100 assays
Detection Method	Absorbance (450 nm)
Applications	Measurement of pyruvate dehydrogenase activity in various tissues/cells Analysis of cell signaling pathway
Features & Benefits	• Simple, rapid & convenient • can measure pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples
Kit Components	• PDH Assay Buffer • PDH Substrate (Lyophilized) • PDH Developer (Lyophilized) • NADH Standard (Lyophilized) • PDH Positive Control
Storage Conditions	-20°C
Shipping Conditions	Gel Pack

引用文獻

Consitt, Leslie A. et al. (2016) Age-related impairments in skeletal muscle PDH phosphorylation and plasma lactate are indicative of metabolic inflexibility and the effects of exercise training. Am J Physiol Endocrinol Metab. 2016 Jul 1;311(1):E145-56.

Luz, Anthony L. et al. (2016) From the Cover: Arsenite Uncouples Mitochondrial Respiration and Induces a Warburg-like Effect in Caenorhabditis elegans. Toxicol Sci. 2016 Aug;152(2):349-62.

Sin et al., Acute Treatment of Resveratrol Alleviates Doxorubicin-Induced Myotoxicity in Aged Skeletal Muscle Through SIRT1-Dependent Mechanisms. J Gerontol A Biol Sci Med Sci, Oct 2015; 10.1093/gerona/glv175.

Hagve et al., Skeletal muscle mitochondria exhibit decreased pyruvate oxidation capacity and increased ROS emission during surgery-induced acute insulin resistance. Am J Physiol Endocrinol Metab, Apr 2015; 308: E613 – E620.

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