單株抗體Clonotyping分型套組-HRP | 貨號5300-05  

 SBA 單株抗體Clonotyping分型套組-HRP試劑盒，專為小鼠單克隆抗體的同型分型而設計。 它含有2.5mg捕獲抗體和1.0mL HRP綴合的抗小鼠Ig，小鼠IgA，小鼠IgG1，小鼠IgG2a，小鼠IgG2b，小鼠IgG3，小鼠IgM，小鼠κ，小鼠λ和ABTS底物。 當與Mouse Immunoglobulin Panel (SB Cat. No. 5300-01)一起使用時，該試劑盒可用於樣品（如血清，上清液和腹水）中小鼠免疫球蛋白的定量研究。The SBA Clonotyping System-C57BL/6-HRP kit is designed for the isotyping of C57BL/6 mouse monoclonal antibodies. It contains 2.5 mg of capture antibody and 1.0 mL of HRP conjugated anti-mouse Ig, mouse IgA, mouse IgG₁, mouse IgG_2b, mouse IgG_2c, mouse IgG₃, mouse IgM, mouse κ, mouse λ, and ABTS substrate. The kit may also be utilized for quantitative studies of mouse immunoglobulins in samples such as serum when used in conjunction with the Mouse Immunoglobulin Panel (SB Cat. No. 5300-01B).

• 使用HRP呈色的ELISA方式鑑別抗體Isotype。
• 用於篩選Hybridomas所產生的抗體，以及鑑定抗體重鍊與輕鍊Isotypes。

產品規格

Goat Anti-Mouse Ig, Human ads-UNLB – 2.5 mg in 1.0 mL
Goat Anti-Mouse Ig, Human ads-HRP – 1.0 mL
Goat Anti-Mouse IgA-HRP – 1.0 mL
Goat Anti-Mouse IgG₁, Human ads-HRP – 1.0 mL
Goat Anti-Mouse IgG_2b, Human ads-HRP – 1.0 mL
Goat Anti-Mouse IgG_2c, Human ads-HRP – 1.0 mL
Goat Anti-Mouse IgG₃, Human ads-HRP – 1.0 mL
Goat Anti-Mouse IgM, Human ads-HRP – 1.0 mL
Goat Anti-Mouse Kappa-HRP – 1.0 mL
Goat Anti-Mouse Lambda-HRP – 1.0 mL
ABTS Substrate Powder – 100 mg

產品型號	產品名稱	包裝
5300-05B	SBA Clonotyping System-HRP	1 Kit
5300-01	Mouse Immunoglobulin Panel	1 Kit
5300-01B	B6/C57J Mouse Immunoglobulin Panel	1 Kit
5300-04	SBA Clonotyping System-AP	1 Kit
5300-04B	SBA Clonotyping System-B6/C57J-AP	1 Kit
5300-05B	SBA Clonotyping System-B6/C57J-HRP	1 Kit

References

1. Tseng T, Chung C. A monoclonal antibody recognizing the specific epitope of aflatoxin analogs. Bot Bull Acad Sin. 1990;31:279-84. (ELISA – Isotyping)
2. Yu L, Lee KK, Ens K, Doig PC, Carpenter MR, Staddon W, et al. Partial characterization of a Candida albicans fimbrial adhesin. Infect Immun. 1994;62:2834-42. (ELISA – Isoyping)
3. Esteban MR, Giovinazzo G, de la Hera A, Goday C. PUMA1: a novel protein that associates with the centrosomes, spindle and centromeres in the nematode Parascaris. J Cell Sci. 1998;111:723-35. (ELISA – Isotyping)
4. Gil OD, Zhang L, Chen S, Ren YQ, Pimenta A, Zanazzi G, et al. Complementary expression and heterophilic interactions between IgLON family members neurotrimin and LAMP. J Neurobiol. 2002;51:190-204. (ELISA – Isotyping)
5. Sánchez MD, Pierson TC, McAllister D, Hanna SL, Puffer BA, Valentine LE, et al. Characterization of neutralizing antibodies to West Nile virus. Virology. 2005;336:70-82. (ELISA – Isotyping)
6. Mazzarotto GA, Raboni SM, Stella V, Carstensen S, de Noronha L, Levis S, et al. Production and characterization of monoclonal antibodies against the recombinant nucleoprotein of Araucaria hantavirus. J Virol Methods. 2009;162:96-100. (ELISA – Isotyping)
7. Wang C, deVries S, Camboni M, Glass M, Martin PT. Immunization with the SDPM1 peptide lowers amyloid plaque burden and improves cognitive function in the APPswePSEN1(A246E) transgenic mouse model of Alzheimer’s disease. Neurobiol Dis. 2010;39:409-22. (ELISA – Isotyping & Serum)
8. Vassiliadis E, Veidal SS, Simonsen H, Larsen DV, Vainer B, Chen X, et al. Immunological detection of the type V collagen propeptide fragment, PVCP-1230, in connective tissue remodeling associated with liver fibrosis. Biomarkers. 2011;16:426-33. (ELISA – Isotyping)
9. Liu Z, Song C, Li Y, Liu F, Zhang K, Sun Y, et al. Development of highly sensitive chemiluminescence enzyme immunoassay based on the anti-recombinant HC subunit of botulinum neurotoxin type A monoclonal antibodies. Anal Chim Acta. 2012;735:23-30. (ELISA – Isotyping)
10. Han Z, Zhao F, Shao Y, Liu X, Kong X, Song Y, et al. Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein. Virus Res. 2013;171:54-64. (ELISA – Isotyping)
11. Wongwairot S, Kueakhai P, Changklungmoa N, Jaikua W, Sansri V, Meemon K, et al. Monoclonal antibody against recombinant Fasciola gigantica cathepsin L1H could detect juvenile and adult cathepsin Ls of Fasciola gigantica. Parasitol Res. 2015;114:133-40. (ELISA – Isotyping)
12. Ren J, Zhu Y, Zhou Y, Lv C, Yan H, Ma L, et al. Identification of three antigen epitopes on the nucleocapsid protein of the genotype C of bovine parainfluenza virus type 3. Vet Microbiol. 2015;178:61-9. (ELISA – Isotyping)
13. Guo C, Tang Y, Qi Z, Liu Y, Zhao X, Huo X, et al. Development and characterization of a panel of cross-reactive monoclonal antibodies generated using H1N1 influenza virus. Immunobiology. 2015;220:941-6. (ELISA – Isotyping)
14. Li C, Liang W, Liu W, Yang D, Wang H, Ma W, et al. Identification of a conserved linear epitope using a monoclonal antibody against non-structural protein 3B of foot-and-mouth disease virus. Arch Virol. 2016;161:365-75. (ELISA – Isotyping)
15. Gu J, Wang L, Jin Y, Lin C, Wang H, Zhou N, et al. Characterization of specific antigenic epitopes and the nuclear export signal of the Porcine circovirus 2 ORF3 protein. Vet Microbiol. 2016;184:40-50. (ELISA – Isotyping)
16. Hansen NU, Willumsen N, Sand JM, Larsen L, Karsdal MA, Lemming DJ. Type VIII collagen is elevated in diseases associated with angiogenesis and vascular remodeling. Clin Biochem. 2016 May 24. doi: 10.1016/j.clinbiochem.2016.05.023. [Epub ahead of print]. (ELISA – Isotyping)
17. Cascalho M, Wong J, Wabl M. VH gene replacement in hyperselected B cells of the quasimonoclonal mouse. J Immunol. 1997;159:5795-801. (ELISA – Serum)
18. Kikuchi T, Hackett NR, Crystal RG. Cross-strain protection against clinical and laboratory strains of Pseudomonas aeruginosa mediated by dendritic cells genetically modified to express CD40 ligand and pulsed with specific strains of Pseudomonas aeruginosa. Hum Gene Ther. 2001;12:1251-63. (ELISA – Serum)
19. Wu X, Jiang N, Deppong C, Singh J, Dolecki G, Mao D, et al. A role for the Cr2 gene in modifying autoantibody production in systemic lupus erythematosus. J Immunol. 2002;169:1587-92. (ELISA – Serum)
20. Ruzek MC, Hawes M, Pratt B, McPherson J, Ledbetter S, Richards SM, et al. Minimal effects on immune parameters following chronic anti-TGF-β monoclonal antibody administration to normal mice. Immunopharmacol Immunotoxicol. 2003;25:235-57. (ELISA – Serum)
21. Olson TS, Bamias G, Naganuma M, Rivera-Nieves J, Burcin TL, Ross W, et al. Expanded B cell population blocks regulatory T cells and exacerbates ileitis in a murine model of Crohn disease. J Clin Invest. 2004;114:389-98. (ELISA – Serum & Supernatant)
22. Coughlin JJ, Stang SL, Dower NA, Stone JC. RasGRP1 and RasGRP3 regulate B cell proliferation by facilitating B cell receptor-Ras signaling. J Immunol. 2005;175:7179-84. (ELISA – Serum)
23. Perchonock CE, Fernando MC, Quinn WJ 3rd, Nguyen CT, Sun J, Shapiro MJ, et al. Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006;26:6005-15. (ELISA – Serum)
24. Travis MA, Reizis B, Melton AC, Masteller E, Tang Q, Proctor JM, et al. Loss of integrin αvβ8 on dendritic cells causes autoimmunity and colitis in mice. Nature. 2007;449:361-5. (ELISA – Serum)
25. Bell JJ, Divekar RD, Ellis JS, Cascio JA, Haymakker CL, Jain R, et al. In trans T cell tolerance diminishes autoantibody responses and exacerbates experimental allergic encephalomyelitis. J Immunol. 2008;180:1508-16. (ELISA – Serum)
26. Bolduc A, Long E, Stapler D, Cascalho M, Tsubata T, Koni PA, et al. Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas. J Immunol. 2010;185:220-30. (ELISA – Serum)
27. Bohgaki T, Bohgaki M, Cardoso R, Panier S, Zeegers D, Li L, et al. Genomic instability, defective spermatogenesis, immunodeficiency, and cancer in a mouse model of the RIDDLE syndrome. PLoS Genet. 2011;7(4):e1001381. (ELISA – Serum & Supernatant)
28. Huang C, Hatzi K, Melnick A. Lineage-specific functions of Bcl-6 in immunity and inflammation are mediated by distinct biochemical mechanisms. Nat Immunol. 2013;14:380-8. (ELISA – Serum)
29. Huang Q, Perlman H, Birkett R, Doyle R, Fang D, Haines GK, et al. CD11c-mediated deletion of Flip promotes autoreactivity and inflammatory arthritis. Nat Commun. 2015;6:7086. (ELISA – Serum)
30. Ying Z, Mei M, Zhang P, Liu C, He H, Gao F, et al. Histone arginine methylation by PRMT7 controls germinal center formation via regulating Bcl6 transcription. J Immunol. 2015;195:1538-47. (ELISA – Serum)
31. Benhamron S, Pattanayak SP, Berger M, Tirosh B. mTOR activation promotes plasma cell differentiation and bypasses XBP-1 for immunoglobulin secretion. Mol Cell Biol. 2015;35:153-66. (ELISA – Serum & Supernatant)
32. Zanotti KJ, Maul RW, Castiblanco DP, Yang W, Choi YJ, Fox JT, et al. ATAD5 deficiency decreases B cell division and Igh recombination. J Immunol. 2015;194:35-42. (ELISA – Serum)
33. Chen J, Li Z, Petersen E, Liu W, Zhu X. Co-administration of interleukins 7 and 15 with DNA vaccine improves protective immunity against Toxoplasma gondii. Exp Parasitol. 2016;162:18-23. (ELISA – Serum)
34. Zhao Y, Li Z, Chen J, Sun X, Liu S, Zhu X, ey al. Protective efficacy of pVAX-RON5p against acute and chronic infections of Toxoplasma gondii in BALB/c mice. Exp Parasitol. 2016;163:24-30. (ELISA – Serum)
35. Corcos D, Iglesias A, Dunda O, Bucchini D, Jami J. Allelic exclusion in transgenic mice expressing a heavy chain disease-like human μ protein. Eur J Immunol. 1991;21:2711-6. (ELISA – Supernatant)
36. Ishida K, Yoshimura K. Characterization of monoclonal antibodies against eosinophil chemotactic factors from young adult worms of Angiostrongylus cantonensis. Parasite Immunol. 1992;14:633-44. (ELISA – Supernatant)
37. Yamada T, Mitani T, Yorita K, Uchida D, Matsushima A, Iwamasa K, et al. Abnormal immune function of hemopoietic cells from alymphoplasia (aly) mice, a natural strain with mutant NF-κB-inducing kinase. J Immunol. 2000;165:804-12. (ELISA – Supernatant)
38. Wittman VP, Woodburn D, Nguyen T, Neethling FA, Wright S, Weidanz JA. Antibody targeting to a class I MHC-peptide epitope promotes tumor cell death. J Immunol. 2006;177:4187-95. (ELISA – Supernatant)
39. Verma B, Hawkins OE, Neethling FA, Caseltine SL, Largo SR, Hildebrand WH, et al. Direct discovery and validation of a peptide/MHC epitope expressed in primary human breast cancer cells using a TCRm monoclonal antibody with profound antitumor properties. Cancer Immunol Immunother. 2010;59:563-73. (ELISA – Supernatant)
40. Zhang L, Zhang H, Fan Z, Zhou X, Yu L, Sun H, et al. Identification of a conserved linear B-cell epitope of Streptococcus dysgalactiae GapC protein by screening phage-displayed random peptide library. PLoS One. 2015;10(6):e0131221. (ELISA – Supernatant & Ascites)
41. Zhang J, Li H, Huo R, Zhai T, Li H, Sun Y, et al. Paeoniflorin selectively inhibits LPS-provoked B-cell function. J Pharmacol Sci. 2015;128:8-16. (ELISA – Supernatant)
42. Zhang L, Zhou X, Fan Z, Tang W, Chen L, Dai J, et al. Identification of a conserved B-cell epitope on the GapC protein of Streptococcus dysgalactiae. Microb Pathog. 2015;83-84:23-8. (ELISA – Supernatant & Ascites)