Fc Silent™ Antibodies: Turning down the background noise 只要訊號不要雜訊 (Engineered Fc Domain)

Anti-GM2 ganglioside抗體 | 貨號Ab00786-23.0

Immunofluorescence staining of fixed MCF7 cells with anti-GM2 ganglioside antibody L55-81 (Ab00786) Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of L55-81 (Ab00786-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing diffuse membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00786-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor® 488 secondary antibody.

L55-81對具有高GM2表達的黑素瘤細胞株( melanoma cell lines)（M14; M24）呈陽性反應，並且與正常細胞類型 (normal cell types) 沒有結合，M15或M25細胞具低GM2表達與高GM3表達。 通過ELISA和TLC免疫染色可以通過測試多種神經節苷脂(gangliosides)和中性醣脂(neutral glycolipids) 來確認這種特異性。 L55-81在人或兔補體存在下, 能夠裂解來自M14黑素瘤細胞系（其具有高GM2表達）的細胞， but not in the absence of complement – this was shown by a [51Cr] release assay。 生物素化的L55-81已用於抗生物素蛋白 – 生物素複合物免疫過氧化物酶系統(avidin-biotin complex immunoperoxidase system) 中丙酮固定(acetone-fixed sections)的腫瘤組織切片的IHC。 用黑色素瘤 ( melanoma)，結腸癌 (colon carcinoma )和卵巢癌腫瘤組織 (ovary carcinoma tumor tissues) 顯示統計學上顯著的陽性染色（determined by Fisher’s exact test）。 在乳腺癌 (breast carcinoma)，前列腺癌 (prostate carcinoma )和腎癌 (kidney carcinoma) 中染色也觀察到此現象。

Species and Isotype: Rabbit IgG, kappa
Clone Number: L55-81
UniProt Accession Number of Target Protein: 
Published Application(s): ELISA; TLC; IHC; depleting
Published Species Reactivity: human
Immunogen: L55-81 was prepared by isolating EBV-immortalized peripheral blood B lymphocytes from a human donor with melanoma.
Specificity: L55-81 binds specifically to GM2 ganglioside; this antibody shows no reactivity towards GM1, GM3, GD2 or normal tissues- the L55-81 epitope contains both a GalNAc and sialic acid bound to lactose. Ganglioside GM2 (GalNAcβ1-4(NeuNAcα2-3)Galβ1-4Glcβ-1ceramide) is a glycosphingolipid present on the cell surface and a major tumor-differentiation antigen in human melanoma and hepatoma.

Antibody first published in: Nishinaka et al. Development of a human monoclonal antibody to ganglioside G(M2) with potential for cancer treatment. Cancer Res. 1996 Dec 15;56(24):5666-71. PMID:8971173
Note on publication: Describes the establishment of an anti-GM2 cell line L55-81 and its action against tumors such as by its anti-tumor activity and its ability to stain tumor sections.

Absoluted Antibody 提供一系列抗體商品，其中Fc Silent™ Antibodies系列；能有效降低非特異性Fc結合。Fc Silent™技術，為應用生物工程技術，將Fc receptors (FcγR, FcR)點突變，進而中止抗體與ADCC的功能；有效降低Fc所造成的背景訊號。Fc Silent™抗體應用於免疫細胞時flow cytometry、immunohistochemistry (IHC)資料顯示，有效降低非特異性之Fc結合，所造成的背景值。(訂購抗體前，請確認選擇抗體是否有Fc Silent™修飾標誌)。